Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.
Department of Medicine, University of Padova, Via Giustiniani 2, 35128 Padova, Italy; Proteomics Center of Padova University, Via G. Orus 2/B, 35129 Padova, Italy.
Food Chem. 2015 Jul 15;179:170-4. doi: 10.1016/j.foodchem.2015.01.112. Epub 2015 Jan 31.
The evaluation of Botrytis cinerea as noble rot on withered grapes is of great importance to predict the wine sensory/organoleptic properties and to manage the winemaking process of Amarone, a passito dry red wine. This report describes the first proteomic analysis of grapes infected by noble rot under withering conditions to identify possible markers of fungal infection. 2-D gel electrophoresis revealed that protein profiles of infected and not infected grape samples are significantly different in terms of number of spots and relative abundance. Protein identification by MS analysis allowed to identify only in infected berries proteins of B. cinerea that represent potential markers of the presence of the fungus in the withered grapes.
灰葡萄孢菌（Botrytis cinerea）作为干枯葡萄上的贵腐菌的评估，对于预测阿玛罗尼（Amarone）风干甜红葡萄酒的葡萄酒感官/感官特性和管理酿酒过程非常重要。本报告描述了在风干条件下感染贵腐菌的葡萄的第一个蛋白质组学分析，以鉴定可能的真菌感染标志物。2-D 凝胶电泳显示，在斑点数量和相对丰度方面，受感染和未受感染的葡萄样品的蛋白质图谱有明显差异。通过 MS 分析进行的蛋白质鉴定仅允许在感染的浆果中鉴定出灰葡萄孢菌的蛋白质，这些蛋白质代表了真菌在风干葡萄中存在的潜在标志物。
