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Second-generation Notch1 activity-trap mouse line (N1IP::CreHI) provides a more comprehensive map of cells experiencing Notch1 activity.第二代Notch1活性捕获小鼠品系（N1IP::CreHI）提供了经历Notch1活性的细胞的更全面图谱。
Development. 2015 Mar 15;142(6):1193-202. doi: 10.1242/dev.119529. Epub 2015 Feb 27.
2
Mapping the consequence of Notch1 proteolysis in vivo with NIP-CRE.利用NIP-CRE在体内绘制Notch1蛋白水解的结果。
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3
[Effect of CRE-dependent RNA interference targeting Notch1 on proliferation of cervical cancer cell line HeLa].[CRE依赖的RNA干扰靶向Notch1对宫颈癌HeLa细胞系增殖的影响]
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4
Generation and characterization of a Notch1 signaling-specific reporter mouse line.Notch1信号特异性报告基因小鼠品系的构建与鉴定
Genesis. 2012 Sep;50(9):700-10. doi: 10.1002/dvg.22030. Epub 2012 May 3.
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In vivo visualization of Notch1 proteolysis reveals the heterogeneity of Notch1 signaling activity in the mouse cochlea.体内可视化 Notch1 蛋白水解揭示了 Notch1 信号活性在小鼠耳蜗中的异质性。
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Cre-conditional expression of constitutively active Notch1 in transgenic mice.转基因小鼠中组成型激活的Notch1的Cre条件性表达。
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Recent advances in Notch signaling measurement.Notch信号通路检测的最新进展。
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Notch1 signaling is limited in healthy mature kidneys in vivo.Notch1 信号在体内健康成熟肾脏中受到限制。
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Notch controls the cell cycle to define leader versus follower identities during collective cell migration.Notch 控制细胞周期，以在细胞集体迁移过程中定义领导者和追随者的身份。
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10
Notch signal strength controls cell fate in the haemogenic endothelium.Notch信号强度控制造血内皮中的细胞命运。
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本文引用的文献

1
Notch signaling regulates ovarian follicle formation and coordinates follicular growth.Notch信号通路调节卵巢卵泡的形成并协调卵泡生长。
Mol Endocrinol. 2014 Apr;28(4):499-511. doi: 10.1210/me.2013-1288. Epub 2014 Feb 19.
2
Asymmetric activation of Dll4-Notch signaling by Foxn4 and proneural factors activates BMP/TGFβ signaling to specify V2b interneurons in the spinal cord.Foxn4 和神经前体细胞因子对 Dll4-Notch 信号的非对称激活作用激活了 BMP/TGFβ 信号，从而在脊髓中特化 V2b 中间神经元。
Development. 2014 Jan;141(1):187-98. doi: 10.1242/dev.092536. Epub 2013 Nov 20.
3
Lack of ADAM10 in endothelial cells affects osteoclasts at the chondro-osseus junction.内皮细胞中 ADAM10 的缺乏会影响软骨-骨交界处的破骨细胞。
J Orthop Res. 2014 Feb;32(2):224-30. doi: 10.1002/jor.22492. Epub 2013 Sep 21.
4
Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle.Notch 通路在小鼠睾丸出生后发育和精子发生周期中的表达动态。
PLoS One. 2013 Aug 28;8(8):e72767. doi: 10.1371/journal.pone.0072767. eCollection 2013.
5
The extracellular domain of Notch2 increases its cell-surface abundance and ligand responsiveness during kidney development.Notch2 的细胞外结构域在肾脏发育过程中增加其细胞表面丰度和配体反应性。
Dev Cell. 2013 Jun 24;25(6):585-98. doi: 10.1016/j.devcel.2013.05.022.
6
Epidermal ADAM17 is dispensable for notch activation.表皮中的ADAM17对于Notch激活并非必需。
J Invest Dermatol. 2013 Sep;133(9):2286-8. doi: 10.1038/jid.2013.162. Epub 2013 Apr 3.
7
Testosterone levels influence mouse fetal Leydig cell progenitors through notch signaling.睾酮水平通过 Notch 信号影响小鼠胎儿睾丸间质祖细胞。
Biol Reprod. 2013 Apr 11;88(4):91. doi: 10.1095/biolreprod.112.106138. Print 2013 Apr.
8
Notch2 is required in somatic cells for breakdown of ovarian germ-cell nests and formation of primordial follicles.Notch2 是体细胞中破坏卵巢生殖细胞巢和形成原始卵泡所必需的。
BMC Biol. 2013 Feb 13;11:13. doi: 10.1186/1741-7007-11-13.
9
Constitutive activation of NOTCH1 signaling in Sertoli cells causes gonocyte exit from quiescence.Sertoli 细胞中 NOTCH1 信号的组成性激活导致精原细胞退出静止状态。
Dev Biol. 2013 May 1;377(1):188-201. doi: 10.1016/j.ydbio.2013.01.031. Epub 2013 Feb 4.
10
Tbx18 targets dermal condensates for labeling, isolation, and gene ablation during embryonic hair follicle formation.Tbx18 靶向真皮凝聚物，用于标记、分离和基因敲除胚胎毛囊形成过程中的基因。
J Invest Dermatol. 2013 Feb;133(2):344-53. doi: 10.1038/jid.2012.329. Epub 2012 Sep 20.

第二代Notch1活性捕获小鼠品系（N1IP::CreHI）提供了经历Notch1活性的细胞的更全面图谱。

Second-generation Notch1 activity-trap mouse line (N1IP::CreHI) provides a more comprehensive map of cells experiencing Notch1 activity.

作者信息

Liu Zhenyi, Brunskill Eric, Boyle Scott, Chen Shuang, Turkoz Mustafa, Guo Yuxuan, Grant Rachel, Kopan Raphael

机构信息

SAGE Labs, St Louis, MO 63146, USA Department of Developmental Biology, Washington University, St Louis, MO 63110, USA.

Division of Developmental Biology, Children's Hospital Medical Center, Cincinnati, OH 45229, USA.

出版信息

Development. 2015 Mar 15;142(6):1193-202. doi: 10.1242/dev.119529. Epub 2015 Feb 27.

DOI:10.1242/dev.119529
PMID:25725069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4360173/
Abstract

We have previously described the creation and analysis of a Notch1 activity-trap mouse line, Notch1 intramembrane proteolysis-Cre6MT or N1IP::Cre(LO), that marked cells experiencing relatively high levels of Notch1 activation. Here, we report and characterize a second line with improved sensitivity (N1IP::Cre(HI)) to mark cells experiencing lower levels of Notch1 activation. This improvement was achieved by increasing transcript stability and by restoring the native carboxy terminus of Cre, resulting in a five- to tenfold increase in Cre activity. The magnitude of this effect probably impacts Cre activity in strains with carboxy-terminal Ert2 fusion. These two trap lines and the related line N1IP::Cre(ERT2) form a complementary mapping tool kit to identify changes in Notch1 activation patterns in vivo as the consequence of genetic or pharmaceutical intervention, and illustrate the variation in Notch1 signal strength from one tissue to the next and across developmental time.

摘要

我们之前描述了一种Notch1活性捕获小鼠品系Notch1膜内蛋白水解-Cre6MT或N1IP::Cre(LO)的构建及分析，该品系可标记经历相对高水平Notch1激活的细胞。在此，我们报告并表征了另一个具有更高敏感性的品系（N1IP::Cre(HI)），用于标记经历较低水平Notch1激活的细胞。这种改进是通过提高转录本稳定性和恢复Cre的天然羧基末端实现的，从而使Cre活性提高了五到十倍。这种效应的大小可能会影响带有羧基末端Ert2融合的品系中的Cre活性。这两个捕获品系以及相关品系N1IP::Cre(ERT2)形成了一个互补的定位工具包，用于识别体内由于基因或药物干预导致的Notch1激活模式的变化，并说明了Notch1信号强度在不同组织之间以及整个发育过程中的差异。