糖谱分配器：用于聚糖液相色谱数据的带误差估计的自动结构分配
GlycoProfileAssigner: automated structural assignment with error estimation for glycan LC data.
作者信息
Duffy Fergal J, Rudd Pauline M
机构信息
National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Co. Dublin, Ireland.
出版信息
Bioinformatics. 2015 Jul 1;31(13):2220-1. doi: 10.1093/bioinformatics/btv129. Epub 2015 Feb 26.
MOTIVATION
Sequencing glycan structures is a difficult problem that requires the use of multiple experimental approaches. One powerful approach to glycan sequencing is the combination of liquid chromatography with sequential exoglycosidase digestions; however, interpreting this can be difficult and time-consuming. To aid this process, we introduce GlycoProfileAssigner, software for automated structural assignment of glycan profile data from liquid chromatography experiments.
RESULTS
GlycoProfileAssigner has been tested on human IgG data, and can retrieve the correct structure in 14 out of 16 peaks tested.
AVAILABILITY AND IMPLEMENTATION
The programme and its source code is available at https://bitbucket.org/fergaljd/glycoprofileassigner
CONTACT
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
动机
对聚糖结构进行测序是一个难题，需要使用多种实验方法。一种强大的聚糖测序方法是液相色谱与外切糖苷酶顺序消化相结合；然而，对此进行解读可能既困难又耗时。为辅助这一过程，我们引入了GlycoProfileAssigner，这是一款用于自动对液相色谱实验中的聚糖谱数据进行结构归属的软件。
结果
GlycoProfileAssigner已在人IgG数据上进行了测试，在测试的16个峰中有14个能检索到正确结构。
可用性与实现方式
该程序及其源代码可在https://bitbucket.org/fergaljd/glycoprofileassigner获取。
联系方式
补充信息
补充数据可在《生物信息学》在线版获取。
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