Xia Bo-Hou, Cao Yi, Xie Wen-Jian, Xu Zhao-Dong, Lin Li-Mei, Liao Duan-Fang
Zhong Yao Cai. 2014 Aug;37(8):1463-6.
To establish a UPLC fingerprint method of Xiasangju Granules.
UPLC analysis was performed on a Waters ACQUITY UPLC H-Class system and carried out at 30 °C on a Waters Column ACQUITY UPLC BEH C18 (2.1 mm x 50 mm, 1.7 μm). A binary gradient elution system was composed of acetonitrile (phase A)and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 320 nm,the mobile flow rate was at 0.4 mL/min. A matrix including 16 variations (characteristic peaks area)and 12 samples was constructed for similarity evaluation, cluster analysis and principle component analysis.
The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 16 characteristic peaks were designated. 12 samples were divided into 6 groups.
It is a reliable, available and quick method for quality control of Xiasangju Granules.
建立夏桑菊颗粒的超高效液相色谱指纹图谱方法。
采用Waters ACQUITY UPLC H-Class系统进行超高效液相色谱分析，色谱柱为Waters Column ACQUITY UPLC BEH C18（2.1 mm×50 mm，1.7 μm），柱温30℃。二元梯度洗脱系统由乙腈（A相）和0.5%乙酸溶液（B相）组成。检测波长为320 nm，流动相流速为0.4 mL/min。构建包含16个变量（特征峰面积）和12个样品的矩阵进行相似度评价、聚类分析和主成分分析。
结果表明所采集的样品相似度良好。生成了特异性指纹图谱，标定了16个特征峰。12个样品分为6组。
该方法是一种可靠、可行且快速的夏桑菊颗粒质量控制方法。
