Hu Y D, Huang Q K, Zhu Q, Lan D, Feng Z Q, Zhang L, Lan X, Ye L, Liu Y P, He M, Pu H B
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University Ya'an, Sichuan, China.
Guangyuan Food of Animal Husbandry Bureau, Guanyuan, Sichuan, China.
Genet Mol Res. 2015 Jan 23;14(1):294-303. doi: 10.4238/2015.January.23.3.
Gonadotropin-inhibitory hormone (GnIH) gene is an important gene in reproduction. In this study, we screened single-nucleotide polymorphisms (SNPs) in the chicken GnIH gene among 204 individuals in Erlang mountainous chickens. We then analyzed the associations between polymorphisms of the GnIH gene and 5 egg production traits in chickens. Five SNPs (T3305C, T3310C, G3403C, G3411A, and T3591C) were detected. Associations between polymorphic loci and age at first egg, body weight at first egg, weight at first egg, egg weight in 300 days, and egg production in 300 days were analyzed using analysis of covariance. The results showed that SNP1, SNP3, and SNP4 had large effects on age at first egg, while SNP5 had a large effect on body weight at first egg; of the effect of the TT genotype was significantly higher than that of CT (P < 0.01). Further analysis show that the highest frequency (0.2353) haplotype H1H1 was associated with the latest age at first egg. The H4H5 haplotype had a positive effect on egg production in 300 days and a negative effect on weight at first egg. We observed no association between the H3H3 haplotype and body weight at first egg.
促性腺激素抑制激素（GnIH）基因是生殖过程中的一个重要基因。在本研究中，我们在二郎山山地鸡的204个个体中筛选了鸡GnIH基因的单核苷酸多态性（SNP）。然后，我们分析了GnIH基因多态性与鸡的5个产蛋性状之间的关联。检测到5个SNP（T3305C、T3310C、G3403C、G3411A和T3591C）。使用协方差分析对多态性位点与开产日龄、开产体重、开产蛋重、300日龄蛋重和300日龄产蛋量之间的关联进行了分析。结果表明，SNP1、SNP3和SNP4对开产日龄影响较大，而SNP5对开产体重影响较大；TT基因型的影响显著高于CT基因型（P<0.01）。进一步分析表明，频率最高（0.2353）的单倍型H1H1与最晚开产日龄相关。单倍型H4H5对300日龄产蛋量有正向影响，对开产体重有负向影响。我们未观察到单倍型H3H
