Zhang L, Li D Y, Liu Y P, Wang Y, Zhao X L, Zhu Q
Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya'an, P.R. China.
Genet Mol Res. 2012 Dec 17;11(4):4307-15. doi: 10.4238/2012.October.2.1.
The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The prolactin (PRLR) receptor is a specific receptor for prolactin, which is an anterior pituitary peptide hormone involved in various physiological activities and is essential for reproductive success. In chickens, the PRLR gene resides on the Z chromosome. We used a pooled DNA sequencing approach for identifying SNPs of the PRLR gene. Three hundred and nine-six Erlang Mountainous chicken hens were genotyped for six SNPs using PCR-SSCP and PCR-sequencing methods; the association with chicken egg production traits was studied using general linear model procedures. Three linked SNPs (G14952A, A14969C and G14984A) at the P1 locus, two linked SNPs (G17560A and T17626A) at the P2 locus, and one SNP (T20868C) at the P3 locus were identified. Eight haplotypes were reconstructed on the basis of the six SNPs. The general linear model analysis indicated that there were significant associations of certain genotypes and haplotypes with some egg production traits. We conclude that chicken PRLR gene polymorphisms are associated with egg production traits and have potential as molecular markers for chicken breeding.
在禽类育种计划中，识别和利用对经济重要性状有显著影响的潜在候选基因变得越来越重要。催乳素（PRLR）受体是催乳素的特异性受体，催乳素是一种腺垂体肽激素，参与各种生理活动，对繁殖成功至关重要。在鸡中，PRLR基因位于Z染色体上。我们采用混合DNA测序方法来识别PRLR基因的单核苷酸多态性（SNP）。使用PCR-SSCP和PCR测序方法对396只二郎山鸡母鸡的6个SNP进行基因分型；使用一般线性模型程序研究其与鸡蛋生产性状的关联。在P1位点鉴定出3个连锁SNP（G14952A、A14969C和G14984A），在P2位点鉴定出2个连锁SNP（G17560A和T17626A），在P3位点鉴定出1个SNP（T20868C）。基于这6个SNP重建了8种单倍型。一般线性模型分析表明，某些基因型和单倍型与一些产蛋性状存在显著关联。我们得出结论，鸡PRLR基因多态性与产蛋性状相关，具有作为鸡育种分子标记的潜力。
