Song Z, Shao D, Sun X X, Niu J W, Gong D Q
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province, China
Genet Mol Res. 2015 Jan 23;14(1):389-99. doi: 10.4238/2015.January.23.12.
Liver weight is an important economic trait in the fatty goose liver industry. Liver-type fatty acid binding protein (L-FABP) is involved in the formation and metabolism of fatty acids. Thus, we hypothesized that sequence polymorphisms in L-FABP were associated with fatty liver weight in goose. We first isolated, sequenced, and characterized the goose L-FABP gene, which had not been previously reported. The goose L-FABP gene was 2490 bp and included 4 exons coding for a 126-amino acid protein. Analysis of expression levels of the goose L-FABP gene in different tissues showed that the expression level in the liver tissue was higher than in other tissues, and was significantly higher in the liver tissue of overfed geese than in control geese. Moreover, a single nucleotide polymorphism located at 774 bp in the gene was identified in a Landes goose population. To test whether this single nucleotide polymorphism was associated with fatty liver production, liver weight and the ratio of liver to carcass weights were determined for the 3 genotypes with this single nucleotide polymorphism (TT, TG, GG) in overfed Landes geese. Our data indicate that individuals with the GG genotype had higher values for the variables measured than those with the other 2 genotypes, suggesting that L-FABP can be a selection marker for the trait of fatty liver production in goose.
肝脏重量是肥肝产业中的一个重要经济性状。肝脏型脂肪酸结合蛋白（L-FABP）参与脂肪酸的形成和代谢。因此，我们推测L-FABP基因的序列多态性与鹅的脂肪肝重量有关。我们首先分离、测序并鉴定了鹅L-FABP基因，该基因此前尚未见报道。鹅L-FABP基因全长2490 bp，包含4个外显子，编码一个由126个氨基酸组成的蛋白质。对鹅L-FABP基因在不同组织中的表达水平分析表明，其在肝脏组织中的表达水平高于其他组织，且在填饲鹅的肝脏组织中显著高于对照鹅。此外，在朗德鹅群体中鉴定出该基因774 bp处的一个单核苷酸多态性。为了检测这个单核苷酸多态性是否与肥肝生产相关，我们测定了填饲朗德鹅中具有该单核苷酸多态性（TT、TG、GG）的3种基因型个体的肝脏重量以及肝脏与胴体重量的比值。我们的数据表明，GG基因型个体的所测变量值高于其他两种基因型个体，这表明L-FABP可作为鹅肥肝生产性状的一个选择标记。
