He H, Liu H H, Wang J W, Lv J, Li L, Pan Z X
Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, 625014, People's Republic of China.
Mol Biol Rep. 2014;41(4):2045-53. doi: 10.1007/s11033-014-3053-5. Epub 2014 Jan 28.
It has been demonstrated that ACSL3 and ACSL5 play important roles in fat metabolism. To investigate the primary functions of ACSL3 and ACSL5 and to evaluate their expression levels during goose fatty liver development, we cloned the ACSL3 and ACSL5 coding domain sequences (CDSs) of geese using RT-PCR and analyzed their expression characteristics under different conditions using qRT-PCR. The results showed that the goose ACSL3 (JX511975) and ACSL5 (JX511976) sequences have high similarities with the chicken sequences both at the nucleotide and amino acid levels. Both ACSL3 and ACSL5 have high expression levels in goose liver. The expression levels of ACSL3 and ACSL5 in goose liver and hepatocytes can be changed by overfeeding geese and by treatment with unsaturated fatty acids, respectively. Together, these results indicate that ACSL3 and ACSL5 play important roles during fatty liver development. The different expression characteristics of goose ACSL3 and ACSL5 suggest that these two genes may be responsible for specific functions.
已证明ACSL3和ACSL5在脂肪代谢中起重要作用。为了研究ACSL3和ACSL5的主要功能，并评估它们在鹅脂肪肝发育过程中的表达水平，我们使用RT-PCR克隆了鹅的ACSL3和ACSL5编码域序列（CDS），并使用qRT-PCR分析了它们在不同条件下的表达特征。结果表明，鹅ACSL3（JX511975）和ACSL5（JX511976）序列在核苷酸和氨基酸水平上与鸡序列具有高度相似性。ACSL3和ACSL5在鹅肝脏中均具有高表达水平。鹅肝脏和肝细胞中ACSL3和ACSL5的表达水平可分别通过过量饲喂鹅和用不饱和脂肪酸处理来改变。总之，这些结果表明ACSL3和ACSL5在脂肪肝发育过程中起重要作用。鹅ACSL3和ACSL5的不同表达特征表明这两个基因可能负责特定功能。
