Li Q, Zhao X L, Gilbert E R, Liu Y P, Wang Y, Qiu M H, Zhu Q
College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan Province, China.
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Genet Mol Res. 2015 Feb 6;14(1):1220-8. doi: 10.4238/2015.February.6.24.
Free-range production system is increasingly being used in poultry breeding and feed production in many countries. The objective of the current experiment was to evaluate the effects of different raising systems on fat-related traits and mRNA levels of liver lipogenesis genes in Erlang Mountainous chicken. Each of 10 birds (91 day old) from caged, indoor-floor housed, and free-range housing systems was slaughtered, and fat-related traits, live body weight (BW), subcutaneous fat thickness (SFT), abdominal fat weight (AFW), abdominal fat percentage (AFP), and intramuscular fat content were determined. The mRNA levels of liver X receptor α, carbohydrate response element-binding protein (ChREBP), sterol regulatory element-binding protein-1 (SREBP1), and fatty acid synthase were detected. The caged chicken exhibited significantly higher BW, SFT, and AFW than those of free-ranged chicken (P < 0.05). All the 4 genes had a similar expression pattern, and they showed the highest level in caged chicken, while the lowest level was found in free-ranged chicken. Association analysis indicated that there were significant (P < 0.05) or highly significant (P < 0.01) positive correlations between the mRNA levels of ChREBP, SREBP1, and fat traits of SFT, AFW, and AFP. Thus, we deduced that increased fat deposition in caged chicken was probably induced by increased gene expression of ChREBP and SREBP1 in the liver.
在许多国家，家禽养殖和饲料生产中越来越多地采用放养生产系统。本实验的目的是评估不同饲养系统对二郎山鸡脂肪相关性状和肝脏脂肪生成基因mRNA水平的影响。从笼养、室内地面饲养和放养系统中各选取10只（91日龄）鸡进行屠宰，测定其脂肪相关性状、活体体重（BW）、皮下脂肪厚度（SFT）、腹脂重量（AFW）、腹脂百分比（AFP）和肌内脂肪含量。检测肝脏X受体α、碳水化合物反应元件结合蛋白（ChREBP）、固醇调节元件结合蛋白-1（SREBP1）和脂肪酸合酶的mRNA水平。笼养鸡的BW、SFT和AFW显著高于放养的鸡（P < 0.05）。这4个基因具有相似的表达模式，在笼养鸡中表达水平最高，而在放养的鸡中最低。关联分析表明，ChREBP、SREBP1的mRNA水平与SFT、AFW和AFP的脂肪性状之间存在显著（P < 0.05）或极显著（P < 0.01）的正相关。因此，我们推断笼养鸡脂肪沉积增加可能是由肝脏中ChREBP和SREBP1基因表达增加所致。
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