Wan Xiaoli, Yang Zhengfeng, Ji Haoran, Li Ning, Yang Zhi, Xu Lei, Yang Haiming, Wang Zhiyue
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Anim Biosci. 2021 Mar;34(3):385-392. doi: 10.5713/ajas.20.0432. Epub 2020 Oct 13.
The present study was conducted to investigate the effects of lycopene on growth performance, abdominal fat deposition, serum lipids levels, activities of hepatic lipid metabolism related enzymes and genes expression in broiler chickens.
A total of 256 healthy one-day-old male Arbor Acres broiler chicks were randomly divided into four groups with eight replicates of eight birds each. Birds were fed basal diet supplemented with 0 (control), 100, 200, and 400 mg/kg lycopene, respectively.
Dietary 100 mg/kg lycopene increased the body weight at 21 day of age compared to the control group (p<0.05). Compared to the basal diet, broilers fed diet with 100 mg/kg lycopene had decreased abdominal fat weight, and broilers fed diet with 100 and 200 mg/kg lycopene had decreased abdominal fat percentage (p<0.05). Compared to control, diets with 100, 200, and 400 mg/kg lycopene reduced the levels of total triglyceride and total cholesterol in serum, and diets with 100 and 200 mg/kg lycopene reduced the level of serum low density lipoprotein cholesterol (p<0.05). The activity of fatty acid synthase (FAS) in 400 mg/kg lycopene treated broilers and the activity of acetyl-CoA carboxylase (ACC) in 100, 200, and 400 mg/kg lycopene treated broilers were lower than those fed basal diet (p<0.05). Lycopene increased the mRNA abundance of adenosine monophosphate activated protein kinase α (AMPK-α), whereas decreased the mRNA abundance of sterol regulatory elementbinding protein 1, FAS, and ACC compared to the control group (p<0.05).
Dietary lycopene supplementation can alleviate abdominal fat deposition and decrease serum lipids levels, possibly through activating the AMPK signaling pathway, thereby regulating lipid metabolism such as lipogenesis. Therefore, lycopene or lycopenerich plant materials might be added to poultry feed to regulate lipid metabolism.
本研究旨在探讨番茄红素对肉鸡生长性能、腹部脂肪沉积、血脂水平、肝脏脂质代谢相关酶活性及基因表达的影响。
选取256只1日龄健康雄性爱拔益加肉鸡雏鸡，随机分为4组，每组8个重复，每个重复8只鸡。分别给鸡饲喂添加0（对照组）、100、200和400mg/kg番茄红素的基础日粮。
与对照组相比，日粮中添加100mg/kg番茄红素可使21日龄时的体重增加（p<0.05）。与基础日粮相比，饲喂100mg/kg番茄红素日粮的肉鸡腹部脂肪重量降低，饲喂100和200mg/kg番茄红素日粮的肉鸡腹部脂肪百分比降低（p<0.05）。与对照组相比，添加100、200和400mg/kg番茄红素的日粮可降低血清总甘油三酯和总胆固醇水平，添加100和200mg/kg番茄红素的日粮可降低血清低密度脂蛋白胆固醇水平（p<0.05）。400mg/kg番茄红素处理的肉鸡中脂肪酸合酶（FAS）活性以及100、200和400mg/kg番茄红素处理的肉鸡中乙酰辅酶A羧化酶（ACC）活性均低于饲喂基础日粮的肉鸡（p<0.05）。与对照组相比，番茄红素增加了腺苷酸活化蛋白激酶α（AMPK-α）的mRNA丰度，而降低了固醇调节元件结合蛋白1、FAS和ACC的mRNA丰度（p<0.05）。
日粮中添加番茄红素可减轻腹部脂肪沉积并降低血脂水平，可能是通过激活AMPK信号通路，从而调节脂质代谢如脂肪生成。因此，可将番茄红素或富含番茄红素的植物性原料添加到家禽饲料中以调节脂质代谢。
