†Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, Shandong 266100, China.
‡Tianjin Branch of Baotou Research Institute of Rare Earth, Tianjin 300300, China.
Environ Sci Technol. 2015 Mar 17;49(6):3761-8. doi: 10.1021/es5056337. Epub 2015 Mar 9.
We fabricated a biomimetic nanofiltration (NF) membrane by immobilizing an Aquaporin Z (AqpZ)-incorporated supported lipid bilayer (SLB) on a layer-by-layer (LbL) complex polyelectrolyte membrane to achieve excellent permeability and salt rejection with a high stability. The polyelectrolyte membranes were prepared by LbL assembly of poly(ethylenimine) (PEI) with positive charges and poly(sodium 4-styrenesulfonate) (PSS) with negative charges alternately on a porous hydrolyzed polyacrylonitrile (H-PAN) substrate. AqpZ-incorporated 1,2-dioleloyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dioleoyl-3-trimethylammo-nium-propane (chloride salt) (DOTAP) vesicles with positive charges were deposited on the H-PAN/PEI/PSS polyelectrolytes membrane surface. The resulting biomimetic membrane exhibited a high flux of 22 L·m(-2)·h(-1) (LMH), excellent MgCl2 rejection of ∼97% and NaCl rejection of ∼75% under an operation pressure of 0.4 MPa. Due to the attractive electrostatic interaction between SLB and the polyelectrolyte membrane, the biomimetic membrane showed satisfactory stability and durability as well as stable NF flux and rejection for at least 36 h. In addition, the AqpZ-containing biomimetic membrane was immersed in a 0.24 mM (critical micellar concentration, CMC) Triton X-100 solution for 5 min. The flux and rejection were slightly influenced by the Triton X-100 treatment. The current investigation demonstrated that the AqpZ-incorporated biomimetic membranes fabricated by the LbL method led to excellent separation performances and robust structures that withstand a high operation pressure for a relatively long time.
我们通过将 Aquaporin Z (AqpZ) 包埋的支撑脂质双层 (SLB) 固定在层层 (LbL) 复合聚电解质膜上来制造仿生纳滤 (NF) 膜，以实现优异的渗透性和盐排斥率，同时具有高稳定性。聚电解质膜是通过带正电荷的聚(乙二胺) (PEI) 和带负电荷的聚(4-苯乙烯磺酸钠) (PSS) 在多孔水解聚丙烯腈 (H-PAN) 基底上交替 LbL 组装而成的。带正电荷的 AqpZ 包埋的 1,2-二油酰基-sn-甘油-3-磷酸胆碱 (DOPC)/1,2-二油酰基-3-三甲基铵丙烷 (氯化物) (DOTAP) 囊泡被沉积在 H-PAN/PEI/PSS 聚电解质膜表面。所得仿生膜在 0.4 MPa 的操作压力下表现出 22 L·m(-2)·h(-1) (LMH) 的高通量，约 97%的 MgCl2 排斥率和约 75%的 NaCl 排斥率。由于 SLB 和聚电解质膜之间的吸引力静电相互作用，仿生膜表现出令人满意的稳定性和耐久性，以及稳定的 NF 通量和排斥率，至少 36 小时。此外，含有 AqpZ 的仿生膜被浸入 0.24 mM (临界胶束浓度，CMC) Triton X-100 溶液中 5 分钟。通量和排斥率受 Triton X-100 处理的影响较小。目前的研究表明，通过 LbL 方法制备的含有 AqpZ 的仿生膜具有出色的分离性能和坚固的结构，能够承受较高的操作压力，持续较长时间。
