Oomori Y, Okuno S, Fujisawa H, Ishikawa K, Satoh Y, Matsuda M, Yamano M, Ono K
Department of Anatomy, Asahikawa Medical College, Japan.
Acta Anat (Basel). 1989;136(1):49-54. doi: 10.1159/000146797.
The present study applied the separated adrenal capsules of rats for wholemount immunocytochemistry and used tyrosine hydroxylase (TH) antibody as a marker for catecholamines. TH-immunoreactive nerve bundles without varicosities and fibers with varicosities were seen to run along or to encircle blood vessels entering the adrenal capsule from the outside, and then to run along a network of blood vessels in the intracapsular region. Also, the TH-immunoreactive nerve bundles and fibers were found to run along blood vessels in the subcapsular region. Some TH-immunoreactive nerve fibers and bundles with varicosities, unassociated with the blood vessels, were seen in the subcapsular region. In this region, TH-immunoreactive nerve fibers with varicosities were often seen to be closely associated with the cortical cells. Some TH-immunoreactive nerve fibers without varicosities were visible within the splanchnic nerve in the subcapsular region. The present study suggests that numerous catecholaminergic nerve fibers are associated with blood vessels forming a network in the superficial region of the rat adrenal gland.
本研究采用大鼠分离的肾上腺被膜进行整装免疫细胞化学实验，并使用酪氨酸羟化酶（TH）抗体作为儿茶酚胺的标志物。观察到无膨体的TH免疫反应性神经束和有膨体的纤维沿着或环绕从外部进入肾上腺被膜的血管走行，然后沿着被膜内区域的血管网络走行。此外，还发现TH免疫反应性神经束和纤维沿着被膜下区域的血管走行。在被膜下区域可见一些与血管无关的有膨体的TH免疫反应性神经纤维和神经束。在该区域，常可见到有膨体的TH免疫反应性神经纤维与皮质细胞紧密相连。在被膜下区域的内脏神经内可见一些无膨体的TH免疫反应性神经纤维。本研究提示，在大鼠肾上腺浅层区域，大量儿茶酚胺能神经纤维与形成网络的血管相关联。
