Oomori Y, Iuchi H, Ishikawa K, Satoh Y, Ono K
Department of Anatomy, Asahikawa Medical College, Japan.
Histochemistry. 1994 Jun;101(5):313-23. doi: 10.1007/BF00268992.
An immunohistochemical and immunoelectron microscopic study was used to demonstrate tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) immunoreactivities in the rat pancreas. Small TH immunoreactive cells were found in close contact with large TH immunonegative ganglion cells among the exocrine glands and were occasionally found in some islets. Some of these TH immunoreactive cells were also DBH immunopositive. The immunoreaction product was seen diffusely in the cytoplasm and in the granule cores of TH immunoreactive cells. All intra-pancreatic ganglion cells were immunoreactive for DBH, but not for TH. The TH immunoreactive cells were identified as small intensely fluorescent (SIF) cells due to their localization and morphological characteristics and showed no insulin, glucagon, somatostatin or pancreatic polypeptide immunoreactivities. These results indicate that SIF cells may release dopamine or noradrenaline to adequate stimuli while the intra-pancreatic ganglion cells with only DBH may not synthesize catecholamines in a normal biosynthetic pathway. TH immunoreactive nerve bundles without varicosities and fibers with varicosities, associated or unassociated with blood vessels, were found in both the exocrine and endocrine pancreas. Close apposition of TH immunoreactive nerve fibers to the smooth muscle and endothelial cells of the blood vessels was observed. A close apposition between TH immunoreactive nerve fibers and exocrine acinar cells and islet endocrine cells was sometimes found in the pancreas. The immunoreaction product was seen diffusely in the axoplasm and in the granular vesicles of the immunoreactive nerve fibers. Since no TH immunoreactive ganglion cells were present in the rat pancreas, the present study suggests that noradrenergic nerve fibers in the pancreas may be extrinsic in origin, and may exert an effect on the regulation of blood flow and on the secretory activity of the acinar cells, duct cells and endocrine cells.
采用免疫组织化学和免疫电子显微镜研究来证明大鼠胰腺中酪氨酸羟化酶（TH）和多巴胺β-羟化酶（DBH）的免疫反应性。在外分泌腺中发现小的TH免疫反应性细胞与大的TH免疫阴性神经节细胞紧密接触，偶尔也在一些胰岛中发现。这些TH免疫反应性细胞中的一些也是DBH免疫阳性的。免疫反应产物在TH免疫反应性细胞的细胞质和颗粒核心中呈弥漫性分布。胰腺内所有神经节细胞对DBH呈免疫反应性，但对TH无反应。由于其定位和形态特征，TH免疫反应性细胞被鉴定为小而强荧光（SIF）细胞，且不显示胰岛素、胰高血糖素、生长抑素或胰多肽免疫反应性。这些结果表明，SIF细胞可能在适当刺激下释放多巴胺或去甲肾上腺素，而仅具有DBH的胰腺内神经节细胞可能无法通过正常生物合成途径合成儿茶酚胺。在胰腺的外分泌部和内分泌部均发现了无膨体的TH免疫反应性神经束以及与血管相关或不相关的有膨体纤维。观察到TH免疫反应性神经纤维与血管的平滑肌和内皮细胞紧密相邻。在胰腺中有时发现TH免疫反应性神经纤维与外分泌腺泡细胞和胰岛内分泌细胞紧密相邻。免疫反应产物在免疫反应性神经纤维的轴浆和颗粒小泡中呈弥漫性分布。由于大鼠胰腺中不存在TH免疫反应性神经节细胞，本研究表明胰腺中的去甲肾上腺素能神经纤维可能起源于外部，并且可能对血流调节以及腺泡细胞、导管细胞和内分泌细胞的分泌活动产生影响。
