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磁泳“邂逅”粘弹性:模块化微流控装置中磁性颗粒的确定性分离

Magnetophoresis 'meets' viscoelasticity: deterministic separation of magnetic particles in a modular microfluidic device.

作者信息

Del Giudice Francesco, Madadi Hojjat, Villone Massimiliano M, D'Avino Gaetano, Cusano Angela M, Vecchione Raffaele, Ventre Maurizio, Maffettone Pier Luca, Netti Paolo A

机构信息

Center for Advanced Biomaterials for Health Care @CRIB, Istituto Italiano di Tecnologia, Largo Barsanti e Matteucci 53, 80125 Naples, Italy.

出版信息

Lab Chip. 2015 Apr 21;15(8):1912-22. doi: 10.1039/c5lc00106d.


DOI:10.1039/c5lc00106d
PMID:25732596
Abstract

The deflection of magnetic beads in a microfluidic channel through magnetophoresis can be improved if the particles are somehow focused along the same streamline in the device. We design and fabricate a microfluidic device made of two modules, each one performing a unit operation. A suspension of magnetic beads in a viscoelastic medium is fed to the first module, which is a straight rectangular-shaped channel. Here, the magnetic particles are focused by exploiting fluid viscoelasticity. Such a channel is one inlet of the second module, which is a H-shaped channel, where a buffer stream is injected in the second inlet. A permanent magnet is used to displace the magnetic beads from the original to the buffer stream. Experiments with a Newtonian suspending fluid, where no focusing occurs, are carried out for comparison. When viscoelastic focusing and magnetophoresis are combined, magnetic particles can be deterministically separated from the original streamflow to the buffer, thus leading to a high deflection efficiency (up to ~96%) in a wide range of flow rates. The effect of the focusing length on the deflection of particles is also investigated. Finally, the proposed modular device is tested to separate magnetic and non-magnetic beads.

摘要

如果磁珠在微流控通道中通过磁泳发生偏转的过程中,这些粒子能以某种方式在设备中沿同一条流线聚焦,那么磁珠的偏转情况将会得到改善。我们设计并制造了一个由两个模块组成的微流控设备,每个模块执行一个单元操作。将磁珠悬浮在粘弹性介质中并输送到第一个模块,该模块是一个直的矩形通道。在这里,利用流体的粘弹性使磁性粒子聚焦。这样的通道是第二个模块(即H形通道)的一个入口,在第二个入口处注入缓冲流。使用永久磁铁将磁珠从原始流位移至缓冲流。为了进行比较,还对不发生聚焦的牛顿悬浮液进行了实验。当将粘弹性聚焦和磁泳相结合时,磁性粒子可以从原始流中确定性地分离到缓冲流中,从而在很宽的流速范围内实现高达约96%的高偏转效率。还研究了聚焦长度对粒子偏转的影响。最后,对所提出的模块化设备进行了测试,以分离磁性和非磁性珠子。

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