Ribeiro Tatiana G, Nascimento André M, Henriques Bárbara O, Chávez-Fumagalli Miguel A, Franca Juçara R, Duarte Mariana C, Lage Paula S, Andrade Pedro H R, Lage Daniela P, Rodrigues Lívia B, Costa Lourena E, Martins Vivian T, Faraco André A G, Coelho Eduardo A F, Castilho Rachel O
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
J Ethnopharmacol. 2015 May 13;165:238-42. doi: 10.1016/j.jep.2015.02.047. Epub 2015 Feb 28.
Stryphnodendron obovatum Benth. is a Brazilian tree used to treat skin ulceration, promote wound healing, and inhibit the growth of protozoa, including Trypanosoma and Leishmania species. Bioguided fractionation of the ethanol extract of S. obovatum stem bark was performed, and antileishmanial and antioxidant activities of the standardized fractions were analyzed.
Stationary-phase Leishmania amazonensis promastigotes, murine macrophages, and human red blood cells (RBCs) were exposed to plant extract, standardized fractions or isolated compounds for 48 h at 37 °C to evaluate their antiparasitic activity and cytotoxicity. The 2,2-diphenyl-1-picryl-hidrazyl assay was used to evaluate antioxidant activity.
The S. obovatum extract and fractions showed antileishmanial and antioxidant activity; however, the organic fraction (OF) showed the best efficacy. We identified gallic acid, gallocatechin, epigallocatechin, catechin, and epigallocatechin gallate in the OF fraction. These compounds effectively inhibited L. amazonensis activity, with gallic acid, gallocatechin, and epigallocatechin gallate showing the highest selectivity. Furthermore, the evaluated compounds had no significant effect on murine macrophages and human RBCs.
The compounds present in the S. obovatum plant bark ethanol extract may provide an alternative therapeutic approach for L. amazonensis treatment.
倒卵叶马钱树（Stryphnodendron obovatum Benth.）是一种巴西树木，用于治疗皮肤溃疡、促进伤口愈合以及抑制包括锥虫和利什曼原虫属在内的原生动物生长。对倒卵叶马钱树茎皮乙醇提取物进行了生物活性导向分离，并分析了标准化组分的抗利什曼原虫活性和抗氧化活性。
将静止期亚马逊利什曼原虫前鞭毛体、小鼠巨噬细胞和人类红细胞（RBC）在37℃下暴露于植物提取物、标准化组分或分离出的化合物中48小时，以评估它们的抗寄生虫活性和细胞毒性。采用二苯基苦味酰基自由基（DPP）法评估抗氧化活性。
倒卵叶马钱树提取物和组分显示出抗利什曼原虫和抗氧化活性；然而，有机组分（OF）显示出最佳效果。我们在OF组分中鉴定出了没食子酸、没食子儿茶素、表没食子儿茶素、儿茶素和表没食子儿茶素没食子酸酯。这些化合物有效地抑制了亚马逊利什曼原虫的活性，没食子酸、没食子儿茶素和表没食子儿茶素没食子酸酯表现出最高的选择性。此外，所评估的化合物对小鼠巨噬细胞和人类红细胞没有显著影响。
倒卵叶马钱树植物树皮乙醇提取物中的化合物可能为亚马逊利什曼原虫的治疗提供一种替代治疗方法。
