Özkoç Soykan, Bayram Delibaş Songül, Erbaycu Ahmet Emin, Ergüden Ceren, Akısü Çiler
Dokuz Eylül Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Türkiye.
Turkiye Parazitol Derg. 2014 Dec;38(4):214-9. doi: 10.5152/tpd.2014.3611.
The detection of Pneumocystis jirovecii or its DNA in respiratory samples from individuals who do not have signs or symptoms of pneumonia has been defined as colonization. In this study, we aimed to investigate the prevalence of P. jirovecii colonization in patients with various lung diseases.
Thirty patients who were followed-up and who had undergone bronchoscopy for diagnosis of different underlying diseases or pulmonary signs were included in the study. Bronchoalveolar lavage (BAL) fluids of these patients were analyzed with nPCR amplification of the mt-LSUrRNA gene of P. jirovecii. In addition to nPCR, giemsa, Gomori's methenamine silver (GMS), and indirect fluorescence antibody (IFA) staining assays were applied to all samples.
P. jirovecii DNA was detected in 21 of 30 (70%) BAL samples by nPCR. However, P. jirovecii cysts were found in 1 of 21 nPCR-positive samples by giemsa and GMS. IFA assay showed six samples to be positive, but only four of them were found to be positive by nPCR.
Result of our study showed that prevalence of P. jirovecii colonization is particularly high in patients with chronic pulmonary diseases, and nPCR was a good assay for evaluation of the colonization of P. jirovecii.
在没有肺炎体征或症状的个体的呼吸道样本中检测耶氏肺孢子菌或其DNA被定义为定植。在本研究中，我们旨在调查各种肺部疾病患者中耶氏肺孢子菌定植的患病率。
本研究纳入了30例接受随访且因诊断不同基础疾病或肺部体征而接受支气管镜检查的患者。对这些患者的支气管肺泡灌洗（BAL）液进行耶氏肺孢子菌mt-LSUrRNA基因的巢式聚合酶链反应（nPCR）扩增分析。除nPCR外，对所有样本均采用吉姆萨染色、Gomori六胺银（GMS）染色和间接荧光抗体（IFA）染色检测。
通过nPCR在30份BAL样本中的21份（70%）中检测到耶氏肺孢子菌DNA。然而，在21份nPCR阳性样本中，通过吉姆萨染色和GMS染色仅在1份样本中发现了耶氏肺孢子菌包囊。IFA检测显示6份样本为阳性，但其中只有4份通过nPCR检测为阳性。
我们的研究结果表明，耶氏肺孢子菌定植的患病率在慢性肺部疾病患者中特别高，并且nPCR是评估耶氏肺孢子菌定植的良好检测方法。
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