Akhond Morteza, Absalan Ghodratollah, Ershadifar Hamid
Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 May 15;143:223-9. doi: 10.1016/j.saa.2015.01.071. Epub 2015 Feb 14.
A novel, simple and highly sensitive colorimetric method is developed for determination of Amoxicillin (AMX). The system is based on aggregation of citrate-capped gold nanoparticles (AuNP) in acetate buffer (pH=4.5) in the presence of the degradation product of Amoxicillin (DPAMX). It was found that the color of gold nanoparticles changed from red to purple and the intensity of surface plasmon resonance (SPR) peak of AuNPs decreased. A new absorption band was appeared in the wavelength range of 600-700nm upon addition of DPAMX. The absorbance ratio at the wavelength of 660 and 525nm (A660/A525) was chosen as the analytical signal indirectly related to AMX concentration. The linearity of the calibration graph was found over the concentration range of 0.3-4.5μM AMX with a correlation coefficient of 0.9967. Under the optimum experimental conditions, the detection limit was found to be 0.15μM. The applicability of the method was successfully demonstrated by analysis of AMX in pharmaceutical formulations including capsules and oral suspensions.
开发了一种新颖、简单且高灵敏度的比色法用于测定阿莫西林（AMX）。该体系基于在醋酸盐缓冲液（pH = 4.5）中，在阿莫西林降解产物（DPAMX）存在下柠檬酸盐包覆的金纳米颗粒（AuNP）的聚集。发现金纳米颗粒的颜色从红色变为紫色，并且AuNPs的表面等离子体共振（SPR）峰强度降低。加入DPAMX后，在600 - 700nm波长范围内出现了一个新的吸收带。选择660和525nm波长处的吸光度比值（A660/A525）作为与AMX浓度间接相关的分析信号。在校准曲线的浓度范围为0.3 - 4.5μM AMX时发现具有线性关系，相关系数为0.9967。在最佳实验条件下，检测限为0.15μM。通过分析包括胶囊和口服混悬液在内的药物制剂中的AMX，成功证明了该方法的适用性。
