Whitfield Jonathan, Littlewood Trevor, Evan Gerard I, Soucek Laura
Vall d'Hebron Institute of Oncology (VHIO), Barcelona 08035, Spain; Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain;
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, United Kingdom.
Cold Spring Harb Protoc. 2015 Mar 2;2015(3):227-34. doi: 10.1101/pdb.top069815.
Reversible regulatory mouse models have significantly contributed to our understanding of normal tissue and cancer biology, providing the opportunity to temporally control initiation, progression, and evolution of physiological and pathological events. The tamoxifen inducible system, one of the best-characterized "reversible switch" models, has a number of beneficial features. In this system, the hormone-binding domain of the mammalian estrogen receptor is used as a heterologous regulatory domain. Upon ligand binding, the receptor is released from its inhibitory complex and the fusion protein becomes functional. We summarize the advantages and drawbacks of the system, describe several mouse models that rely on it, and discuss potential improvements that could render it even more useful and versatile.
可逆调控小鼠模型对我们理解正常组织和癌症生物学做出了重大贡献，为暂时控制生理和病理事件的起始、进展和演变提供了机会。他莫昔芬诱导系统是特征最明确的“可逆开关”模型之一，具有许多有益特性。在该系统中，哺乳动物雌激素受体的激素结合结构域被用作异源调控结构域。配体结合后，受体从其抑制复合物中释放出来，融合蛋白变得具有功能。我们总结了该系统的优缺点，描述了几种依赖于它的小鼠模型，并讨论了可能使其更有用和更通用的潜在改进。
