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利用钾离子通道的孔模块构建一种对Ca⁺⁺⁺敏感的（生物）传感器。

Engineering a Ca⁺⁺⁺-sensitive (bio)sensor from the pore-module of a potassium channel.

作者信息

DiFrancesco Mattia Lorenzo, Gazzarrini Sabrina, Arrigoni Cristina, Romani Giulia, Thiel Gerhard, Moroni Anna

机构信息

Department of Biosciences, University of Milan, Via Celoria 26, Milano 20133, Italy.

Institute of Biophysics-Milan section, National Research Council, Via Celoria 26, Milano 20133, Italy.

出版信息

Sensors (Basel). 2015 Feb 27;15(3):4913-24. doi: 10.3390/s150304913.

DOI:10.3390/s150304913
PMID:25734643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4435187/
Abstract

Signals recorded at the cell membrane are meaningful indicators of the physiological vs. pathological state of a cell and will become useful diagnostic elements in nanomedicine. In this project we present a coherent strategy for the design and fabrication of a bio-nano-sensor that monitors changes in intracellular cell calcium concentration and allows an easy read out by converting the calcium signal into an electrical current in the range of microampere that can be easily measured by conventional cell electrophysiology apparatus.

摘要

在细胞膜上记录的信号是细胞生理状态与病理状态的重要指标，并且将成为纳米医学中有用的诊断元件。在本项目中，我们提出了一种连贯的策略，用于设计和制造一种生物纳米传感器，该传感器可监测细胞内钙离子浓度的变化，并通过将钙信号转换为微安级的电流实现轻松读数，而这种电流可通过传统的细胞电生理仪器轻松测量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/a2dab9814466/sensors-15-04913-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/ea0fbdd83500/sensors-15-04913-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/6a523014235a/sensors-15-04913-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/8fcb4104a916/sensors-15-04913-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/b99d17196438/sensors-15-04913-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/a2dab9814466/sensors-15-04913-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/ea0fbdd83500/sensors-15-04913-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/6a523014235a/sensors-15-04913-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/8fcb4104a916/sensors-15-04913-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/b99d17196438/sensors-15-04913-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f0c/4435187/a2dab9814466/sensors-15-04913-g005.jpg

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