一种针对阿尔茨海默病淀粉样前体蛋白细胞外结构域的抗血清可识别70 kDa和88 kDa的脑蛋白。

An antiserum to the extracellular domain of the Alzheimer amyloid precursor recognizes 70 and 88 kDa brain proteins.

作者信息

Simpson J, Bladon C M, Yates C M, Harmar A J

机构信息

Department of Pharmacology, University of Edinburgh, Scotland.

出版信息

FEBS Lett. 1989 Nov 6;257(2):238-40. doi: 10.1016/0014-5793(89)81542-x.

DOI:10.1016/0014-5793(89)81542-x

PMID:2573541

Abstract

An antiserum raised to the extracellular domain (residues 556-566) of the Alzheimer amyloid precursor protein recognized 70 and 88 kDa proteins in Western blots of rat, Alzheimer, Down's syndrome and control human brain separated by SDS-PAGE. The 70 kDa protein band was resolved into 5 spots by two-dimensional electrophoresis. The findings provide further evidence that a 70 kDa protein is a metabolite of the amyloid precursor protein and reveal an 88 kDa protein which was reduced in 3 out of 6 brains with Alzheimer pathology.

摘要

一种针对阿尔茨海默病淀粉样前体蛋白细胞外结构域（第556 - 566位氨基酸残基）制备的抗血清，在经SDS-PAGE分离的大鼠、患阿尔茨海默病的人、唐氏综合征患者及对照人脑的Western印迹中识别出70 kDa和88 kDa的蛋白质。通过二维电泳，70 kDa的蛋白条带被分离为5个斑点。这些发现进一步证明70 kDa蛋白质是淀粉样前体蛋白的一种代谢产物，并揭示了一种88 kDa的蛋白质，在6例有阿尔茨海默病病理改变的大脑中有3例该蛋白含量减少。