Fujii H
First Departent of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1989 Jul;64(4):420-30.
Class II subregions of rat major histocompatibility complex (RT1) consist of RT1. B (HLA-DQ equivalent), D (HLA-DR equivalent), and a newly defined H (HLA-DP equivalent) subregions, arranged in the order of RT1.A (class I-H-B beta (beta 1, beta 2)-D (alpha, beta) RFLP analyses of RT1. B and D subregions confirmed that RFLP patterns were correlated well with the allelic specificities which have been determined by serology or MLR. RT1.B locus of BDIX was shown to have RT1.Bd. RFLF analyses of intra-RT1 recombinant rats revealed that recombinations occurred at the sites between RT1.H and B in NIG III, WIN, KGH, between RT1.A and H in DA. BI, BDIX, and between RT1. H alpha and H beta in LEJ. Taken together, the order of genes around H subregion was determined as follows: RT1.A (class I)-H beta-H alpha-B-. Expressions of H subregion genes has not been known. However, Northern hybridization analysis with DP alpha probe detected mRNA of approximately 1.7 kb, suggesting the transcription of RT1. H alpha gene.
大鼠主要组织相容性复合体（RT1）的Ⅱ类亚区由RT1.B（相当于HLA - DQ）、D（相当于HLA - DR）和一个新定义的H（相当于HLA - DP）亚区组成，按RT1.A（Ⅰ类）- H - Bβ（β1，β2）- D（α，β）的顺序排列。对RT1.B和D亚区的RFLP分析证实，RFLP模式与通过血清学或混合淋巴细胞反应确定的等位基因特异性密切相关。BDIX的RT1.B位点显示有RT1.Bd。对RT1内重组大鼠的RFLP分析表明，在NIG III、WIN、KGH中，重组发生在RT1.H和B之间的位点；在DA、BI、BDIX中，重组发生在RT1.A和H之间；在LEJ中，重组发生在RT1.Hα和Hβ之间。综合起来，H亚区周围基因的顺序确定如下：RT1.A（Ⅰ类）- Hβ - Hα - B -。H亚区基因的表达情况尚不清楚。然而，用DPα探针进行的Northern杂交分析检测到约1.7kb的mRNA，提示RT1.Hα基因发生了转录。
