Matsuno Y
Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1987 Sep;62(5):771-8.
In the present study, genomic DNAs obtained from intra-RT1 recombinant rats were subjected to restriction fragment length polymorphism (RELP) analysis, using HLA-DP beta cDNA as a probe. Out of 8 strains of rats whose haplotypes were evaluated serologically, 3 strains (NIG-III, LEJ, and DA. 1I (BI)), including 1 congenic strain, had RT1. A/B recombinant haplotypes (RT1. AqB1D1, AuBbDb, and AnBaDa, respectively). After digestion with BglII or HincII, RFLP analysis of these strains with HLA-DP beta probe showed strong bands associated with RT 1. A haplotypes, in addition to some weaker cross-hybridizing bands. That is, after BglII digestion, 4.2kb band was detected associated with RT 1. Aq, 2.1 kb band with A1 and Ab, 4.3 kb band with Au, respectively. After HincII digestion, 1.2 kb band was detected in association with RT1. Aa and 13.0 kb band with An, respectively. From these results I conclude that the recombination events have occurred within the class II region of RT1, especially between B beta gene and a novel class II beta gene which is proposed to be mapped near RT1. A region. The novel gene seems to be a candidate for DP beta-like gene in RT1, because it strongly hybridized with HLA-DP beta cDNA. As in the mouse or the man, a recombinational hotspot might be generated around this novel gene, which provides another evidence for the structural homology among the MHCs of these three species.
在本研究中，以HLA - DPβ cDNA为探针，对从RT1内部重组大鼠获得的基因组DNA进行限制性片段长度多态性（RELP）分析。在通过血清学评估单倍型的8个大鼠品系中，3个品系（NIG - III、LEJ和DA.1I（BI）），包括1个同源近交系，具有RT1. A/B重组单倍型（分别为RT1. AqB1D1、AuBbDb和AnBaDa）。用BglII或HincII消化后，用HLA - DPβ探针对这些品系进行RFLP分析，结果显示除了一些较弱的交叉杂交带外，还有与RT1. A单倍型相关的强带。也就是说，BglII消化后，检测到与RT1. Aq相关的4.2kb条带、与A1和Ab相关的2.1kb条带、与Au相关的4.3kb条带。HincII消化后，分别检测到与RT1. Aa相关的1.2kb条带和与An相关的13.0kb条带。根据这些结果，我得出结论，重组事件发生在RT1的II类区域内，特别是在Bβ基因和一个新的II类β基因之间，该新基因被认为定位于RT1. A区域附近。这个新基因似乎是RT1中DPβ样基因的一个候选基因，因为它与HLA - DPβ cDNA强烈杂交。与小鼠或人类一样，在这个新基因周围可能产生了一个重组热点，这为这三个物种的MHC之间的结构同源性提供了另一个证据。
