Badave Gitanjali Kailas, Kulkarni Dhananjay
Assistant Professor, Department of Microbiology, Konaseema Institute of Medical Science & Research Foundation , Amalapuram, Andhra Pradesh, India .
Assistant Professor, Department of Pharmacology, Konaseema Institute of Medical Science & Research Foundation , Amalapuram, Andhra Pradesh, India .
J Clin Diagn Res. 2015 Jan;9(1):DC08-10. doi: 10.7860/JCDR/2015/11014.5398. Epub 2015 Jan 1.
To study the quantitative method for biofilm formation and examine the correlation between biofilm formation and antibiotic resistance among the clinical isolates of Acinetobacter baumannii.
A total of 72 A. baumannii isolates from different clinical specimens were processed and confirmed by conventional microbiological methods. Antimicrobial susceptibility testing was performed by Kirby Bauer disc diffusion method using six antibiotics. Biofilm formation was studied by microtitre plate assay.
Forty five (62.5%) of 72 isolates produced biofilm. Resistance to ampicillin-sulbactam was least. 36.1% isolates were resistant to imipenem, 66.6% to ceftazidime, 72.2% to ciprofloxacin, 80.5% to amikacin and 84.7% to piperacillin. Biofilm formers showed greater resistance to ampicillin- sulbactam, amikacin, ciprofloxacin and ceftazidime as compared to imipenem and piperacillin. In all 65 (90.3%) isolates showed multiple drug resistance. Correlation between multidrug resistance and biofilm formation was analysed statistically and p-value was found to be significant (p-value =0.0004; p-value < 0.05 is significant by Chi - Square Test).
The study concludes that there a positive correlation between biofilm formation and multiple drug resistance in A. baumannii.
研究鲍曼不动杆菌临床分离株生物膜形成的定量方法，并检测生物膜形成与抗生素耐药性之间的相关性。
对从不同临床标本中分离出的72株鲍曼不动杆菌进行处理，并采用传统微生物学方法进行鉴定。使用六种抗生素，通过 Kirby Bauer 纸片扩散法进行药敏试验。采用微量滴定板法研究生物膜形成情况。
72株分离株中有45株（62.5%）形成生物膜。对氨苄西林 - 舒巴坦的耐药率最低。36.1%的分离株对亚胺培南耐药，66.6%对头孢他啶耐药，72.2%对环丙沙星耐药，80.5%对阿米卡星耐药，84.7%对哌拉西林耐药。与亚胺培南和哌拉西林相比，生物膜形成菌对氨苄西林 - 舒巴坦、阿米卡星、环丙沙星和头孢他啶表现出更高的耐药性。所有分离株中，65株（90.3%）表现出多重耐药性。对多重耐药性与生物膜形成之间的相关性进行统计学分析，发现p值具有显著性（p值 = 0.0004；卡方检验中p值 < 0.05具有显著性）。
该研究得出结论，鲍曼不动杆菌生物膜形成与多重耐药性之间存在正相关。
