Yoshida Shuhei, Sugahara Kazuma, Hashimoto Makoto, Hirose Yoshinobu, Shimogori Hiroaki, Yamashita Hiroshi
Department of Otolaryngology, Yamaguchi University Graduate School of Medicine , Ube, Yamaguchi , Japan.
Acta Otolaryngol. 2015 May;135(5):411-5. doi: 10.3109/00016489.2014.979438. Epub 2015 Mar 5.
Our data indicate that SSSR and SSSR + FGLM-NH2 protect sensory hair cells against neomycin-induced death in the vestibular epithelium. In addition, the results show that SSSR and FGLM-NH2 can be used as protective molecules against aminoglycoside ototoxicity.
This study investigated the role of the peptides SSSR and SSSR + FGLM-NH2 in mammalian vestibular hair cell death induced by aminoglycoside.
Cultured utricles from mature CBA/N mice were used in this study. The cultured utricles were assigned to five groups (control group, neomycin group, neomycin + SSSR group, neomycin + FGLM-NH2 group, and neomycin + SSSR + FGLM-NH2 group). Aat 24 h after exposure to neomycin, the hair cells were labeled immunohistochemically, and the rate of survival of vestibular hair cells was evaluated using a fluorescence microscope.
The rate of survival of vestibular hair cells was significantly higher in the neomycin + SSSR and neomycin + SSSR + FGLM-NH2 groups than in the neomycin group. The results suggest that SSSR could protect hair cells against aminoglycoside ototoxicity.
我们的数据表明，SSSR和SSSR + FGLM-NH2可保护感觉毛细胞免受新霉素诱导的前庭上皮细胞死亡。此外，结果表明，SSSR和FGLM-NH2可作为抗氨基糖苷类耳毒性的保护分子。
本研究调查了肽SSSR和SSSR + FGLM-NH2在氨基糖苷类诱导的哺乳动物前庭毛细胞死亡中的作用。
本研究使用来自成熟CBA/N小鼠的培养椭圆囊。将培养的椭圆囊分为五组（对照组、新霉素组、新霉素+ SSSR组、新霉素+ FGLM-NH2组和新霉素+ SSSR + FGLM-NH2组）。在接触新霉素24小时后，对毛细胞进行免疫组织化学标记，并使用荧光显微镜评估前庭毛细胞的存活率。
新霉素+ SSSR组和新霉素+ SSSR + FGLM-NH2组前庭毛细胞的存活率显著高于新霉素组。结果表明，SSSR可以保护毛细胞免受氨基糖苷类耳毒性。
