Li Y, Liu X F
Wei Sheng Wu Xue Bao. 1989 Oct;29(5):348-53.
A panel of twelve hybridoma cell lines, secreting specific antibodies to K88 adhesin antigens of enterotoxigenic Escherichia coli (ETEC) were established from eight separate fusions between mouse myeloma cell line Sp 2/0-Ag-14 and spleen cells from mice immunized with purified K88 antigens. Among the 12 monoclonal antibodies (MCA), K-A, K-35, K-11, and K-15 were K88a specific and reacted with all K88 adhesin bearing Escherichia coli strains tested, whatever K88ab, K88ac or K88ad they might be, as shown either in enzyme-linked immunosorbent assay (ELISA) or in direct agglutination test, whereas K32, K-4, and K-3 were specific for G88ab, K88ac, and K88ad respectively. The antigen patterns of 33 K88 bearing Escherichia coli strains covering 3 serotypes of K88ab, K88ac, and K88ad were analyzed by the use of these MCAs. The preliminary results showed that all Escherichia strains with the same serotype of K88 antigen shared at least one common type-specific antigenic determinant, that K88ad and K88ac strains enjoyed one common antigenic determinant that did not exist on K88ab strains, and that there were a few K88 antigenic determinants that appeared only on limited Escherichia coli strains of the same K88 serotype.
用纯化的K88抗原免疫小鼠，取其脾细胞与小鼠骨髓瘤细胞系Sp 2/0-Ag-14进行8次单独融合，建立了一组12个杂交瘤细胞系，这些细胞系分泌针对产肠毒素大肠杆菌（ETEC）K88黏附素抗原的特异性抗体。在这12种单克隆抗体（MCA）中，K-A、K-35、K-11和K-15对K88a具有特异性，并且与所有测试的携带K88黏附素的大肠杆菌菌株发生反应，无论它们是K88ab、K88ac还是K88ad，这在酶联免疫吸附测定（ELISA）或直接凝集试验中均有显示，而K32、K-4和K-3分别对G88ab、K88ac和K88ad具有特异性。利用这些单克隆抗体分析了33株携带K88的大肠杆菌菌株的抗原模式，这些菌株涵盖K88ab、K88ac和K88ad的3种血清型。初步结果表明，所有具有相同血清型K88抗原的大肠杆菌菌株至少共享一种共同的型特异性抗原决定簇，K88ad和K88ac菌株具有一种K88ab菌株所没有的共同抗原决定簇，并且存在一些仅出现在相同K88血清型的有限大肠杆菌菌株上的K88抗原决定簇。
