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利用纯化的K88ab和K88ad抗原制备特异性大肠杆菌K88抗血清。

Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.

作者信息

Guinée P A, Mooi F R, Jansen W H

出版信息

Zentralbl Bakteriol A. 1980 Nov;248(2):182-9.

PMID:6784381
Abstract

Escherichia coli K88ab and K88ad antigens were isolated from K88 antigen-producing E. coli K12 transconjugants and purified by means of repeated column chromatography. Injection of about 1 to 2 mg of the purified antigens into rabbits yielded specific K88ab and K88ad antisera. Attempts to obtain specific K88ac antiserum by means of this procedure failed.

摘要

从产生K88抗原的大肠杆菌K12转接合子中分离出大肠杆菌K88ab和K88ad抗原，并通过反复柱色谱法进行纯化。向兔子注射约1至2毫克纯化抗原后，获得了特异性的K88ab和K88ad抗血清。通过该方法获得特异性K88ac抗血清的尝试未成功。

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Preparation of specific Escherichia coli K88 antisera by means of purified K88ab and K88ad antigens.利用纯化的K88ab和K88ad抗原制备特异性大肠杆菌K88抗血清。
Zentralbl Bakteriol A. 1980 Nov;248(2):182-9.
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[Monoclonal antibodies specific for K88ab, K88ac and K88ad antigens of Escherichia coli].[针对大肠杆菌K88ab、K88ac和K88ad抗原的单克隆抗体]
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PCR assay for detection and differentiation of K88ab(1), K88ab(2), K88ac, and K88ad fimbrial adhesins in E. coli strains isolated from diarrheic piglets.用于检测和区分从腹泻仔猪分离的大肠杆菌菌株中K88ab(1)、K88ab(2)、K88ac和K88ad菌毛粘附素的聚合酶链反应检测法
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