Yoon Se Young, Hong Min Eui, Chang Won Seok, Sim Sang Jun
Department of Chemical and Biological Engineering, Korea University, Seoul, 136-713, South Korea.
Bioprocess Biosyst Eng. 2015 Jul;38(7):1415-21. doi: 10.1007/s00449-015-1383-x. Epub 2015 Mar 5.
Under autotrophic conditions, highly productive biodiesel production was achieved using a semi-continuous culture system in Neochloris oleoabundans. In particular, the flue gas generated by combustion of liquefied natural gas and natural solar radiation were used for cost-effective microalgal culture system. In semi-continuous culture, the greater part (~80%) of the culture volume containing vegetative cells grown under nitrogen-replete conditions in a first photobioreactor (PBR) was directly transferred to a second PBR and cultured sequentially under nitrogen-deplete conditions for accelerating oil accumulation. As a result, in semi-continuous culture, the productivities of biomass and biodiesel in the cells were increased by 58% (growth phase) and 51% (induction phase) compared to the cells in batch culture, respectively. The semi-continuous culture system using two stage photobioreactors is a very efficient strategy to further improve biodiesel production from microalgae under photoautotrophic conditions.
在自养条件下，使用半连续培养系统在富油新绿藻中实现了高产生物柴油生产。特别是，液化天然气燃烧产生的烟道气和自然太阳辐射被用于具有成本效益的微藻培养系统。在半连续培养中，在第一个光生物反应器（PBR）中在氮充足条件下生长的含有营养细胞的大部分培养体积（约80%）直接转移到第二个PBR中，并在氮耗尽条件下依次培养以加速油脂积累。结果，与分批培养的细胞相比，在半连续培养中，细胞中生物质和生物柴油的生产率分别提高了58%（生长阶段）和51%（诱导阶段）。使用两级光生物反应器的半连续培养系统是在光合自养条件下进一步提高微藻生物柴油产量的非常有效的策略。
