Verduci Lorena, Azzalin Gianluca, Gioiosa Silvia, Carissimi Claudia, Laudadio Ilaria, Fulci Valerio, Macino Giuseppe
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Dipartimento di Biotecnologie Cellulari ed Ematologia, "Sapienza" Università di Roma, Roma, Italy.
Leuk Res. 2015 Apr;39(4):479-85. doi: 10.1016/j.leukres.2015.01.010. Epub 2015 Jan 28.
Acute lymphoblastic leukemia (ALL) is an aggressive cancer that occurs in both children and adults. Starting from an integrated analysis of miRNA/mRNA expression profiles in 20 ALL patients, we identify a negative correlation between miR-181a and EGR1. Coherently, miR-181a over-expression in Jurkat T-ALL cells decreases EGR1 expression, increasing cell proliferation and enhancing the cell-cycle progression from G1 to S phase. We show that EGR1 is a new direct target of miR-181a. Our findings suggest that miR-181a behaves as an onco-miRNA in ALL by down-regulating EGR1.
急性淋巴细胞白血病（ALL）是一种侵袭性癌症，在儿童和成人中均可发生。从对20例ALL患者的miRNA/mRNA表达谱进行综合分析开始，我们发现miR-181a与EGR1之间呈负相关。连贯地，Jurkat T-ALL细胞中miR-181a的过表达降低了EGR1的表达，增加了细胞增殖并促进了从G1期到S期的细胞周期进程。我们表明EGR1是miR-181a的一个新的直接靶点。我们的研究结果表明，miR-181a通过下调EGR1在ALL中发挥癌基因miRNA的作用。
