Faculty of Material Science and Ceramics, AGH University of Science and Technology, Mickiewicza 30, 30059 Cracow, Poland.
J Anal Methods Chem. 2015;2015:626458. doi: 10.1155/2015/626458. Epub 2015 Feb 9.
A glassy carbon electrode modified with a Nafion/MWCNTs composite is shown to enable the determination of codeine using differential pulse voltammetry in phosphate buffer of pH 3.0. At a preconcentration time of 15 s, the calibration graph is linear in the 0.5 µM (0.15 mg·L(-1)) to 15 µM (4.5 mg·L(-1)) concentration range with a correlation coefficient of 0.998. The detection limit at a preconcentration time of 120 s is as low as 4.5 μg·L(-1). The repeatability of the method at a 0.6 μg·L(-1) concentration level, expressed as the RSD, is 3.7% (for n = 5). The method was successfully applied and validated by analyzing codeine in drug, human plasma, and urine samples.
一种修饰有 Nafion/MWCNTs 复合材料的玻碳电极,被证明可以通过在 pH 值为 3.0 的磷酸盐缓冲液中使用差分脉冲伏安法来测定可待因。在预浓缩时间为 15s 的情况下,校准曲线在 0.5μM(0.15mg·L(-1))至 15μM(4.5mg·L(-1))浓度范围内呈线性关系,相关系数为 0.998。在预浓缩时间为 120s 的情况下,检测限低至 4.5μg·L(-1)。在 0.6μg·L(-1)浓度水平下,该方法的重复性(表示为 RSD)为 3.7%(n = 5)。该方法成功地应用于药物、人血浆和尿液样品中可待因的分析,并得到了验证。