Bargiel Izabela, Smajdor Joanna, Górska Anna, Paczosa-Bator Beata, Piech Robert
Department of Analytical Chemistry and Biochemistry, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza, 30-059 Kraków, Poland.
Materials (Basel). 2021 Dec 9;14(24):7582. doi: 10.3390/ma14247582.
A new voltametric method for highly sensitive propranolol (PROP) determination was developed. A glassy carbon electrode modified with a hybrid material made of carbon black (CB) and Nafion was used as the working electrode. The preconcentration potential and time were optimized (550 mV and 15 s), as well as the supporting electrolyte (0.1 mol L HSO). For 15 s preconcentration time, linearity was achieved in the range 0.5-3.5 μmol L and for 120 s in 0.02-0.14 μmol L. Based on the conducted calibration (120 s preconcentration time) limit of detection (LOD) was calculated and was equal to 7 nmol L. To verify the usefulness of the developed method, propranolol determination was carried out in real samples (tablets and freeze-dried urine). Recoveries were calculated and were in the range 92-102%, suggesting that the method might be considered as accurate. The repeatability of the signal expressed as relative standard deviation (RSD) was equal to 1.5% ( = 9, PROP concentration 2.5 µmol L). The obtained results proved that the developed method for propranolol determination might be successfully applied in routine laboratory practice.
开发了一种用于高灵敏度测定普萘洛尔（PROP）的新伏安法。用由炭黑（CB）和Nafion制成的混合材料修饰的玻碳电极作为工作电极。对预富集电位和时间（550 mV和15 s）以及支持电解质（0.1 mol L HSO）进行了优化。对于15 s的预富集时间，线性范围为0.5 - 3.5 μmol L，对于120 s的预富集时间，线性范围为0.02 - 0.14 μmol L。基于所进行的校准（120 s预富集时间）计算出检测限（LOD），其等于7 nmol L。为验证所开发方法的实用性，在实际样品（片剂和冻干尿液）中进行了普萘洛尔的测定。计算回收率，回收率在92 - 102%范围内，表明该方法可认为是准确的。以相对标准偏差（RSD）表示的信号重复性等于1.5%（ = 9，PROP浓度2.5 µmol L）。所得结果证明，所开发的普萘洛尔测定方法可成功应用于常规实验室实践。
