Jerris Robert C, Williams Sally R, MacDonald Heather J, Ingebrigtsen Danielle R, Westblade Lars F, Rogers Beverly B
Children's Healthcare of Atlanta, Atlanta, Georgia, USA Emory University School of Medicine, Atlanta, Georgia, USA.
Children's Healthcare of Atlanta, Atlanta, Georgia, USA.
J Clin Pathol. 2015 May;68(5):394-6. doi: 10.1136/jclinpath-2014-202833. Epub 2015 Mar 5.
The FilmArray Respiratory Panel (RP) detects multiple pathogens, including Bordetella pertussis. The multiplex PCR system is appropriate for a core laboratory or point of care due to ease of use. The purpose of this study is to compare the analytical sensitivity of the FilmArray RP, which targets the promoter region of the B. pertussis toxin gene, with the Focus real-time PCR assay, which targets the insertion sequence IS481.
Seventy-one specimens from patients aged 1 month to 18 years, which had tested positive for B. pertussis using the Focus assay, were analysed using the FilmArray RP.
Forty-six specimens were positive for B. pertussis by both the Focus and the FilmArray RP assays. Twenty-five specimens were negative for B. pertussis using the FilmArray RP assay, but positive using the Focus assay.
The FilmArray RP assays will detect approximately 1/3 less cases of B. pertussis than the Focus assay.
FilmArray呼吸道检测板(RP)可检测多种病原体,包括百日咳博德特氏菌。由于使用方便,该多重PCR系统适用于核心实验室或即时检测。本研究的目的是比较靶向百日咳博德特氏菌毒素基因启动子区域的FilmArray RP与靶向插入序列IS481的Focus实时PCR检测法的分析灵敏度。
对71例年龄在1个月至18岁之间、使用Focus检测法检测百日咳博德特氏菌呈阳性的患者标本进行FilmArray RP分析。
Focus检测法和FilmArray RP检测法均显示46例标本百日咳博德特氏菌呈阳性。25例标本使用FilmArray RP检测法百日咳博德特氏菌呈阴性,但使用Focus检测法呈阳性。
FilmArray RP检测法检测到的百日咳博德特氏菌病例比Focus检测法少约1/3。