[液相色谱-串联质谱法同时分析水果、蔬菜、糙米和乌龙茶中的残留农药]

[Simultaneous analysis of residual pesticides in fruit, vegetables, brown rice, and oolong tea by LC-MS/MS].

作者信息

Namikawa Mikio, Shibata Sayo, Shiomi Tetsuo, Nakagawa Tomoyuki, Ban Soichiro, Tomita Yoko, Semura Shunsuke, Nakao Yoshie, Banno Yukinori, Kawakami Masahiro

机构信息

Kyoto City Institute of Health and Environmental Science.

出版信息

Shokuhin Eiseigaku Zasshi. 2014;55(6):279-89. doi: 10.3358/shokueishi.55.279.

DOI:10.3358/shokueishi.55.279

PMID:25743591

Abstract

According to "The validity evaluation guideline of the testing methodology on the residual pesticides in food" of the Ministry of Health, Labour and Welfare of Japan, we carried out validity evaluation of a simultaneous analysis method for residual pesticides in various products with the QuEChERS method using a C18 mini column and/or an EnviCarb/PSA mini column for purification. Multi-ingredient simultaneous analysis with high sensitivity in a short time was achieved with LC-MS/MS. Validation was achieved for 269 pesticides among 302 pesticides in eight kinds of fruit, vegetables, and brown rice and 204 pesticides among 233 pesticides in oolong tea. Finally, 151 pesticides in oolong tea and from 234 to 259 pesticides in fruit, vegetables, and brown rice met the values specified in the guideline.

摘要

根据日本厚生劳动省《食品中残留农药检测方法的有效性评估指南》，我们使用C18微型柱和/或EnviCarb/PSA微型柱进行净化，采用QuEChERS方法对各类产品中的残留农药同时分析方法进行了有效性评估。利用液相色谱-串联质谱法（LC-MS/MS）在短时间内实现了多成分高灵敏度同时分析。对8种水果、蔬菜和糙米中的302种农药中的269种以及乌龙茶中的233种农药中的204种进行了验证。最终，乌龙茶中的151种农药以及水果、蔬菜和糙米中的234至259种农药符合指南规定的值。