Sato Tamaki, Miyamoto Iori, Uemura Masako, Nakatani Tadashi, Kakutani Naoya, Yamano Tetsuo
Osaka City Institute of Public Health and Environmental Sciences.
Shokuhin Eiseigaku Zasshi. 2016;57(4):107-15. doi: 10.3358/shokueishi.57.107.
A validation study was carried out on a rapid method for the simultaneous determination of pesticide residues in vegetables and fruits by LC-MS/MS. Preparation of the test solution was performed by a solid-phase extraction technique with QuEChERS (STQ method). Pesticide residues were extracted with acetonitrile using a homogenizer, followed by salting-out and dehydration at the same time. The acetonitrile layer was purified with C18 and PSA mini-columns. The method was assessed for 130 pesticide residues in 14 kinds of vegetables and fruits at the concentration level of 0.01 μg/g according to the method validation guideline of the Ministry of Health, Labour and Welfare of Japan. As a result 75 to 120 pesticide residues were determined satisfactorily in the tested samples. Thus, this method could be useful for a rapid and simultaneous determination of multi-class pesticide residues in various vegetables and fruits.
对一种通过液相色谱-串联质谱法同时测定蔬菜和水果中农药残留的快速方法进行了验证研究。测试溶液的制备采用了基于QuEChERS的固相萃取技术（STQ方法）。使用匀浆器用乙腈提取农药残留，同时进行盐析和脱水。乙腈层用C18和PSA微型柱进行净化。根据日本厚生劳动省的方法验证指南，在浓度水平为0.01μg/g时对14种蔬菜和水果中的130种农药残留进行了该方法的评估。结果，在测试样品中令人满意地测定了75至120种农药残留。因此，该方法可用于快速同时测定各种蔬菜和水果中的多类农药残留。
