Zhang Rui-Li, Wang Qian-Qiu, Zhang Jing-Ping, Yang Li-Jia
Department of Dermatology, Wuxi Second Affiliated Hospital of Nanjing Medical University, Wuxi, Jiangsu Province, China.
Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing, Jiangsu Province, China; Peking Union Medical College, Nanjing, Jiangsu Province, China; National Center for STD Control, China Centers for Diseases Control and Prevention, Nanjing, Jiangsu Province, China.
Int Immunopharmacol. 2015 Apr;25(2):538-44. doi: 10.1016/j.intimp.2015.02.028. Epub 2015 Mar 2.
Tp17, a membrane immunogen of Treponema pallidum subsp. pallidum, was initially recognized as an inflammatory mediator of syphilis. Because the histopathology of syphilis is characterized by endothelial cell abnormalities, we investigated the effects of recombinant Tp17 (rTp17) on endothelial cell activation in vitro. Using real-time reverse transcription-PCR and whole-cell ELISA, we found that rTp17 activated endothelial cells, as demonstrated by the up-regulated expression and increased gene transcription of intercellular adhesion molecule 1 (ICAM-1), E-selectin, and monocyte chemoattractant protein-1 (MCP-1). rTp17 also enhanced the migration and subsequent adhesion of monocytes to endothelial cells as well as increased transendothelial migration of monocytes. These data suggest that the ability of Tp17 to activate endothelial cells may play an important role in the immunopathogenesis of syphilis.
苍白密螺旋体亚种的膜免疫原Tp17最初被认为是梅毒的一种炎症介质。由于梅毒的组织病理学特征为内皮细胞异常，我们在体外研究了重组Tp17（rTp17）对内皮细胞活化的影响。通过实时逆转录聚合酶链反应和全细胞酶联免疫吸附测定，我们发现rTp17可激活内皮细胞，表现为细胞间黏附分子1（ICAM-1）、E-选择素和单核细胞趋化蛋白-1（MCP-1）的表达上调和基因转录增加。rTp17还增强了单核细胞向内皮细胞的迁移及随后的黏附，以及单核细胞的跨内皮迁移。这些数据表明，Tp17激活内皮细胞的能力可能在梅毒的免疫发病机制中起重要作用。
