Kłębukowska Lucyna, Zadernowska Anna, Chajęcka-Wierzchowska Wioleta
Department of Industrial and Food Microbiology, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-726 Olsztyn, Poland.
J Food Sci Technol. 2015 Mar;52(3):1802-7. doi: 10.1007/s13197-013-1169-6. Epub 2013 Sep 25.
Garlic is valued more for its flavoring and used in a wide variety of foods. In food technology, fresh garlic is not used, but instead its processed forms, most often dried and lyophilized, are utilized. The quality and safety of the final product largely depends on their microbiological quality. This research has provided information about effect of garlic fixation methods and provided information about effect of microbiological contamination of garlic used as a spice for quality of garlic mayonnaise sauce. The authors decided to undertake studies following a report from one of the manufacturers of garlic sauces on product defects which originated in dried garlic used in the production process. Samples of garlic (n = 26) were examinated using standard cultural methods (counts of fungi, lactic acid bacteria-LAB, spore-producing Bacillus sp. and the presence of anaerobic saccharolytic and proteolytic clostridia), automated system TEMPO (total viable count, Enterobacteriaceae), immunoenzymatic method using VIDAS tests (occurrence of Salmonella sp. and Listeria monocytogenes). The number of total viable count was ranged from 3.51 to 6.85 log CFU/g. Enterobacteriaceae were detected only in one sample. Comparably low values were recorded for fungi (1.30 to 3.47 log CFU/g). The number of LAB was ranged from 2.34 to 5.49 log CFU/g. Clostridium sp. were detected in 22 samples. Salmonella sp. and Listeria monocytogenes were not detected. It was found that garlic, regardless of th preservation procedure, might be a source of contamination of garlic mayonnaise sauce especially with lactic acid bacteria and Clostridium sp. spores.
大蒜因其调味作用而备受重视,并被广泛用于各种食品中。在食品技术中,不使用新鲜大蒜,而是使用其加工形式,最常见的是干燥和冻干形式。最终产品的质量和安全性在很大程度上取决于其微生物质量。本研究提供了有关大蒜固定方法的影响的信息,并提供了有关用作大蒜蛋黄酱调味料的大蒜微生物污染对其质量影响的信息。作者根据一家大蒜酱制造商关于生产过程中使用的干大蒜导致产品缺陷的报告决定开展研究。使用标准培养方法(真菌、乳酸菌-LAB、产芽孢芽孢杆菌属计数以及厌氧糖分解和蛋白水解梭菌的存在)、自动化系统TEMPO(总活菌数、肠杆菌科)、使用VIDAS测试的免疫酶法(沙门氏菌属和单核细胞增生李斯特氏菌的存在)对大蒜样本(n = 26)进行检测。总活菌数范围为3.51至6.85 log CFU/g。仅在一个样本中检测到肠杆菌科。真菌的记录值相对较低(1.30至3.47 log CFU/g)。乳酸菌数量范围为2.34至5.49 log CFU/g。在22个样本中检测到梭菌属。未检测到沙门氏菌属和单核细胞增生李斯特氏菌。研究发现,无论采用何种保存程序,大蒜都可能是大蒜蛋黄酱污染的来源,尤其是乳酸菌和梭菌属孢子污染。