Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere 33101, Finland.
Center of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2015 Sep;191:369-76. doi: 10.1016/j.biortech.2015.02.075. Epub 2015 Feb 26.
The aim of this study was to select a potential microalgal strain for lipid production and to examine the suitability of anaerobically treated piggery wastewater as a nutrient source for production of lipid-rich biomass with the selected microalga. Biomass and lipid productivity of three microalgal strains (Chlorella sorokiniana CY1, Chlorella vulgaris CY5 and Chlamydomonas sp. JSC-04) were compared by using different media, nitrogen sources, and nitrogen concentrations. The highest lipid content and productivity (62.5 wt%, 162 mg/L/d) were obtained with C. vulgaris with BG-11 with 62 mg N/L. Secondly, C. vulgaris was cultivated in sterilized, diluted (1-20×), anaerobically treated piggery wastewater. Biomass production decreased and lipid content increased, when wastewater was more diluted. The highest lipid content of 54.7 wt% was obtained with 20× dilution, while the highest lipid productivity of 100.7 mg/L/d with 5× dilution. Piggery wastewater is a promising resource for mass production of oleaginous microalgal biomass.
本研究旨在选择一种潜在的产油微藻菌株，并研究厌氧处理的养猪废水作为生产富油生物量的营养源的适宜性，所选择的微藻。通过使用不同的培养基、氮源和氮浓度，比较了三种微藻菌株（Chlorella sorokiniana CY1、Chlorella vulgaris CY5 和 Chlamydomonas sp. JSC-04）的生物量和脂质生产力。用 BG-11 培养基和 62 毫克/升氮获得了最高的脂质含量和生产力（62.5 重量%，162 毫克/升/天）。其次，将 C. vulgaris 在消毒、稀释（1-20 倍）的厌氧处理养猪废水中进行培养。当废水稀释度更高时，生物量生产减少，脂质含量增加。在 20 倍稀释时获得了最高的 54.7%的脂质含量，而在 5 倍稀释时获得了最高的 100.7 毫克/升/天的脂质生产力。养猪废水是大规模生产油脂微藻生物量的有前途的资源。
