利用双载体进行小球藻 AK-1 的半批量培养，以有效处理高浓度养猪废水。
Semi-batch cultivation of Chlorella sorokiniana AK-1 with dual carriers for the effective treatment of full strength piggery wastewater treatment.
机构信息
University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan.
出版信息
Bioresour Technol. 2021 Apr;326:124773. doi: 10.1016/j.biortech.2021.124773. Epub 2021 Jan 28.
In this study, process optimization for the microalgae-based piggery wastewater treatment was carried out by growing Chlorella sorokiniana AK-1 on untreated piggery wastewater with efficient COD/BOD/TN/TP removal and high biomass/protein productivities. Integration of the immobilization carriers (sponge, activated carbon) and semi-batch cultivation resulted in the effective treatment of raw untreated piggery wastewater. With 100% wastewater, 0.2% sponge and 2% activated carbon, the semi-batch cultivation (90% media replacement every 6 days) exhibited a COD, BOD, TN and TP removal efficiency of 95.7%, 99.0%, 94.1% and 96.9%, respectively. The maximal protein content, protein productivity, lutein content, and lutein productivity of the obtained microalgal biomass was 61.1%, 0.48 g/L/d, 4.56 mg/g, and 3.56 mg/L/d, respectively. The characteristics of the treated effluent satisfied Taiwan Piggery Wastewater Discharge Standards (COD < 600 mg/L, BOD < 80 mg/L). This innovative approach demonstrated excellent performance for simultaneous piggery wastewater treatment and microalgal biomass production.
本研究通过在未经处理的养猪废水中培养普通小球藻 AK-1，实现了基于微藻的养猪废水处理的工艺优化，具有高效 COD/BOD/TN/TP 去除率和高生物质/蛋白质生产力。固定化载体（海绵、活性炭）与半分批培养相结合，实现了对原始未经处理的养猪废水的有效处理。在 100%废水、0.2%海绵和 2%活性炭的条件下，半分批培养（每 6 天更换 90%的培养基）对 COD、BOD、TN 和 TP 的去除效率分别达到 95.7%、99.0%、94.1%和 96.9%。获得的微藻生物质的最大蛋白含量、蛋白生产力、叶黄素含量和叶黄素生产力分别为 61.1%、0.48g/L/d、4.56mg/g 和 3.56mg/L/d。处理后废水的特性符合台湾养猪废水排放标准（COD<600mg/L，BOD<80mg/L）。这种创新方法在同时进行养猪废水处理和微藻生物质生产方面表现出了优异的性能。
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