Dalloul A H, Mossalayi M D, Dellagi K, Bertho J M, Debré P
Laboratoire d'Immunologie, Hôpital Pitié-Salpêtrière, Paris, France.
Eur J Immunol. 1989 Nov;19(11):1985-90. doi: 10.1002/eji.1830191103.
Human CD2+CD3-CD4-CD8- thymocytes were shown to display high in vitro growth ability although their factor requirements for activation and proliferation are not fully known. We have thus isolated these precursors and assayed their activation and proliferation potentials in response to various factors. Our results indicate that these cells proliferate in response to phytohemagglutinin (PHA), recombinant interleukin 2 (rIL 2) and rIL 4. Simultaneous addition of anti-CD2I + III monoclonal antibodies (mAb) and rIL 2 highly increased cell growth while IL 4-induced proliferation was not enhanced upon addition of anti-CD2. Anti-CD2 and PHA, but not IL 2, induced intracytoplasmic Ca2+ influx phosphatidyl inositol turnover as well as IL 2 receptor expression. Sequential studies indicated that CD2 triggering enable many more CD2+ precursors to respond to rIL 2. Endogenous IL 2 synthesis was necessary for PHA-induced cell growth. Neither of these in vitro treatment were able to induce membrane expression of CD3, CD4 or CD8 on CD2+ cells.
人CD2⁺CD3⁻CD4⁻CD8⁻胸腺细胞虽对激活和增殖的因子需求尚不完全清楚，但已显示出较高的体外生长能力。因此，我们分离了这些前体细胞，并检测了它们对各种因子的激活和增殖潜能。我们的结果表明，这些细胞对植物血凝素（PHA）、重组白细胞介素2（rIL-2）和rIL-4有增殖反应。同时添加抗CD2Ⅰ+Ⅲ单克隆抗体（mAb）和rIL-2可显著提高细胞生长，而添加抗CD2后，IL-4诱导的增殖并未增强。抗CD2和PHA可诱导胞质内Ca²⁺内流、磷脂酰肌醇周转以及IL-2受体表达，但IL-2无此作用。连续研究表明，CD2触发可使更多CD2⁺前体细胞对rIL-2产生反应。内源性IL-2合成对于PHA诱导的细胞生长是必需的。这些体外处理均不能诱导CD2⁺细胞表面表达CD3、CD4或CD8。
