Lorré K, Van Damme J, Verwilghen J, Baroja M L, Ceuppens J L
Department of Medicine and Pathophysiology, University of Leuven, Belgium.
J Immunol. 1990 Jun 15;144(12):4681-7.
Recent studies have demonstrated that IL-1 and IL-6 are synergistic accessory signals for activation of T cells. In this study, highly purified human T cells were cultured with either a stimulating pair of anti-CD2 mAb or with immobilized anti-CD3 mAb. Monocytes, a cellfree monocyte culture supernatant or IL-1 were required for anti-CD2-stimulated T cell proliferation, and they each strongly enhanced anti-CD3-induced T cell growth. IL-6 was synergistic with IL-1 as a helper factor for T cell growth after activation via CD2, but we could not demonstrate any effect of IL-6 in the CD3 pathway. The mechanism of the synergistic helper activity of IL-1 and IL-6 on T cell activation in the CD2 pathway was further examined. IL-1 (but not IL-6) was required for induction of IL-2 production. Both IL-1 and IL-6 enhanced IL-2R (p55) expression and the proliferative response to IL-2. T cell proliferation after stimulation with anti-CD2 and IL-1 or IL-1/IL-6 proceeded through an autocrine IL-2-dependent pathway. Moreover we found that, in the absence of IL-1, IL-6 still supported a transient and limited proliferation of anti-CD2- (but not of anti-CD3-) stimulated T cells, which apparently was independent of the autocrine growth factors IL-2 or IL-4. Our data suggest that IL-6 is important as an accessory signal for T cell growth in the CD2 pathway of T cell activation.
最近的研究表明，白细胞介素-1（IL-1）和白细胞介素-6（IL-6）是T细胞激活的协同辅助信号。在本研究中，将高度纯化的人T细胞与一对刺激型抗CD2单克隆抗体（mAb）或固定化抗CD3 mAb一起培养。抗CD2刺激的T细胞增殖需要单核细胞、无细胞单核细胞培养上清液或IL-1，并且它们各自都能强烈增强抗CD3诱导的T细胞生长。IL-6与IL-1协同作用，作为通过CD2激活后T细胞生长的辅助因子，但我们未能证明IL-6在CD3途径中有任何作用。进一步研究了IL-1和IL-6在CD2途径中对T细胞激活的协同辅助活性机制。诱导IL-2产生需要IL-1（而非IL-6）。IL-1和IL-6均增强IL-2受体（p55）表达以及对IL-2的增殖反应。用抗CD2和IL-1或IL-1/IL-6刺激后的T细胞增殖通过自分泌IL-2依赖性途径进行。此外，我们发现，在没有IL-1的情况下，IL-6仍然支持抗CD2（而非抗CD3）刺激的T细胞短暂且有限的增殖，这显然独立于自分泌生长因子IL-2或IL-4。我们的数据表明，IL-6作为T细胞激活CD2途径中T细胞生长的辅助信号很重要。
