巴西驴、骡和马中抗刚地弓形虫抗体的出现及其分离和基因分型
Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil.
作者信息
Gennari Solange M, Esmerini Patrícia de O, Lopes Marcos G, Soares Herbert S, Vitaliano Sérgio N, Cabral Aline D, Pena Hilda F J, Horta Maurício C, Cavalcante Paulo H, Fortes Kleber P, Villalobos Eliana M C
机构信息
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.
出版信息
Vet Parasitol. 2015 Apr 15;209(1-2):129-32. doi: 10.1016/j.vetpar.2015.01.023. Epub 2015 Feb 4.
The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.
在来自巴西不同地区的驴、骡和马中测定了抗刚地弓形虫抗体的出现情况。通过间接荧光抗体试验（临界值 = 64）对304头驴（67.11%）、118匹马（26.05%）和31头骡（6.84%）的血清样本进行了分析。在129头马属动物（28.47%）（82头驴、32匹马和15头骡）中检测到了抗刚地弓形虫抗体。为了通过小鼠生物测定法分离寄生虫，从19只血清阳性和50只血清阴性动物中获取了组织样本，并且从一头驴中分离出了刚地弓形虫。通过使用11个基因标记的聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）对分离株进行基因分型鉴定，确定其为基因型#163（TgCkBr220），该基因型已在巴西的鸡中被描述过。
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