Sasamoto Takeo, Kobayashi Maki, Sakai Naoko, Kamijo Kyoko, Ootani Harunori, Hayashi Masaki, Baba Itoko, Hanashiro Chikako, Takano Ichiro
Tokyo Metropolitan Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2015;56(1):19-30. doi: 10.3358/shokueishi.56.19.
We performed a single-laboratory validation study of a simple and simultaneous determination method for pesticide residues in meat using LC-MS/MS. Water was added to the sample and the mixture was homogenized. Next, pesticides were extracted with acetonitrile containing 1 vol% formic acid using a homogenizer, and salted out with magnesium sulfate, trisodium citrate and sodium chloride. After centrifugation, the acetonitrile layer was made up to standard volume and analyzed by LC-MS/MS. This method was assessed by performing recovery tests in retail bovine, swine and chicken muscle samples spiked with the 132 pesticides at the levels of 0.01 and 0.04 μg/g. Among them, 125 pesticides satisfied the Japanese method validation guideline criteria in bovine, 120 in swine and 127 in chicken.
我们使用液相色谱-串联质谱法（LC-MS/MS）对一种简单同时测定肉类中农药残留的方法进行了单实验室验证研究。向样品中加入水并将混合物均质化。接下来，使用均质器用含1体积%甲酸的乙腈提取农药，并用硫酸镁、柠檬酸三钠和氯化钠进行盐析。离心后，将乙腈层定容至标准体积并通过LC-MS/MS进行分析。通过在零售的牛、猪和鸡肉肌肉样品中添加132种农药，添加水平为0.01和0.04μg/g，进行回收率测试来评估该方法。其中，125种农药在牛肉中符合日本方法验证指南标准，120种在猪肉中符合，127种在鸡肉中符合。
