State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
J Chromatogr A. 2014 Apr 25;1339:103-9. doi: 10.1016/j.chroma.2014.02.084. Epub 2014 Mar 6.
Boronate affinity chromatography (BAC) is a unique means for the selective separation and enrichment of 1,2 and 1,3 cis-diol-containing compounds. However, conventional boronate affinity materials require a basic binding pH (usually≥8.5), which gives rise to not only inconvenience in operation but also the risk of degradation of labile compounds. Although the applicable pH has been expanded to 5.0 in recent years, the current boronate affinity materials still fail to meet the acidic pH end of frequently used biosamples, particularly urine (pH 4.5). In this study, we report a 3-pyridylboronic acid-functionalized organic-silica hybrid monolithic capillary that exhibited a binding pH of 4.5, the lowest so far in BAC. Such a binding pH enabled direct extraction of cis-diol-containing biomolecules such as nucleosides from urine samples without pH adjustment. The boronate affinity monolithic capillary showed enhanced affinity toward negatively charged cis-diol-containing analytes such as ribonucleotides. Moreover, it could function as an anion exchanger at acidic pH (∼2). The column was found to retain multiple compounds from urine, which can be assumed to be at least mostly if not entirely cis-diol-containing compounds.
硼酸亲和层析（BAC）是一种独特的方法，用于选择性分离和富集含有 1,2 和 1,3 顺式二醇的化合物。然而，传统的硼酸亲和材料需要碱性结合 pH（通常≥8.5），这不仅操作不便，而且还存在不稳定化合物降解的风险。尽管近年来适用的 pH 值已扩展到 5.0，但目前的硼酸亲和材料仍无法满足常用生物样品（尤其是尿液，pH4.5）的酸性 pH 值端。在本研究中，我们报告了一种 3-吡啶硼酸功能化的有机-硅杂化整体毛细管，其结合 pH 值为 4.5，是迄今为止 BAC 中最低的。如此低的结合 pH 值可实现核苷等含有顺式二醇的生物分子无需 pH 调整即可直接从尿液样品中提取。硼酸亲和整体毛细管对带负电荷的含有顺式二醇的分析物（如核苷酸）表现出增强的亲和力。此外，它在酸性 pH（约 2）下可作为阴离子交换剂。该柱可从尿液中保留多种化合物，这些化合物至少可以假定为顺式二醇化合物。
