Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
J Chromatogr A. 2009 Nov 20;1216(47):8421-5. doi: 10.1016/j.chroma.2009.10.014. Epub 2009 Oct 13.
Boronate affinity chromatography is an important tool for specific isolation of cis-diol-containing compounds such as glycoproteins, RNA and carbohydrates. Boronate functionalized monolithic capillaries have been recently developed for specific capture of cis-diol-containing small biomolecules, but the apparent hydrophobicity of the columns prevents them from specific capture of glycoproteins. In this paper, a hydrophilic boronate affinity monolithic capillary was prepared by in situ free radical polymerization, using 4-vinylphenylboronic acid (VPBA) and N, N'-methylenebisacrylamide (MBAA) as functional monomer and cross-linker, respectively. The prepared poly(VPBA-co-MBAA) monolithic capillary exhibited uniform open channel network and high density of accessible boronic acid. Due to the utilization of hydrophilic cross-linker, the prepared column was hydrophilic, allowing for specific capture of glycoproteins.
硼酸亲和层析是一种用于特异性分离含有顺式二醇的化合物（如糖蛋白、RNA 和碳水化合物）的重要工具。硼酸功能化整体毛细管最近已被开发用于特异性捕获含有顺式二醇的小分子生物分子，但由于柱体的明显疏水性，它们无法特异性捕获糖蛋白。在本文中，通过原位自由基聚合制备了亲水性硼酸亲和整体毛细管，分别使用 4-乙烯基苯硼酸（VPBA）和 N，N'-亚甲基双丙烯酰胺（MBAA）作为功能单体和交联剂。所制备的聚（VPBA-co-MBAA）整体毛细管具有均匀的开放通道网络和高密度的可及硼酸。由于使用了亲水性交联剂，制备的柱体具有亲水性，允许特异性捕获糖蛋白。
