Pantasri Tawiwan, Wu Linda L, Hull M Louise, Sullivan Thomas R, Barry Michael, Norman Robert J, Robker Rebecca L
The Robinson Research Institute, School of Paediatrics & Reproductive Health, University of Adelaide, Adelaide, SA 5005, Australia.
School of Population Health, University of Adelaide, Adelaide, SA 5005, Australia.
Reprod Fertil Dev. 2015 May;27(4):593-601. doi: 10.1071/RD14321.
Obesity is associated with decreased pregnancy rates due, in part, to compromised oocyte quality. The aim of the present cross-sectional study of 84 women undergoing oocyte aspiration was to: (1) compare insulin, lipids and glucose in follicular fluid with serum; (2) determine whether increased body mass index (BMI) and waist circumference, hyperinsulinaemia, dyslipidaemia or metabolic syndrome altered follicular fluid metabolites; and (3) determine relative lipid content in oocytes to reveal any influence of these parameters on oocyte quality and IVF outcomes. Insulin, glucose, triglyceride and free fatty acids were lower in follicular fluid than blood and not strictly correlated between compartments. Insulin, glucose and triglyceride positively correlated with increasing BMI and waist circumference in blood and follicular fluid. Insulin increased in follicular fluid in association with metabolic syndrome. Free fatty acid composition analysis showed saturated fatty acids, particularly palmitic and stearic acid, to be more prevalent in follicular fluid than blood. There were no associations between follicular fluid metabolites or oocyte lipid content and clinical outcomes; however, oocyte immaturity correlated with follicular fluid glucose and fatty acid levels, as well as metabolic syndrome. The present study confirms that the human ovarian follicular environment surrounding the oocyte exhibits a unique metabolite profile compared with blood, with distinct localisation of lipids within follicular fluid and oocytes.
肥胖与妊娠率降低有关，部分原因是卵母细胞质量受损。本横断面研究对84名接受卵母细胞抽吸的女性进行，目的是：（1）比较卵泡液和血清中的胰岛素、脂质和葡萄糖；（2）确定体重指数（BMI）增加、腰围增加、高胰岛素血症、血脂异常或代谢综合征是否会改变卵泡液代谢物；（3）确定卵母细胞中的相对脂质含量，以揭示这些参数对卵母细胞质量和体外受精结果的任何影响。卵泡液中的胰岛素、葡萄糖、甘油三酯和游离脂肪酸低于血液，且各区间之间无严格相关性。血液和卵泡液中的胰岛素、葡萄糖和甘油三酯与BMI和腰围增加呈正相关。卵泡液中的胰岛素随着代谢综合征而增加。游离脂肪酸组成分析表明，饱和脂肪酸，尤其是棕榈酸和硬脂酸，在卵泡液中比在血液中更普遍。卵泡液代谢物或卵母细胞脂质含量与临床结果之间无关联；然而，卵母细胞不成熟与卵泡液葡萄糖和脂肪酸水平以及代谢综合征相关。本研究证实，与血液相比，围绕卵母细胞的人类卵巢卵泡环境呈现出独特的代谢物谱，脂质在卵泡液和卵母细胞中具有不同的定位。
