Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Science, Huzhou University, Huzhou, PR China.
Department of Animal Sciences and Aquatic Ecology, Ghent University, Ghent, Belgium.
PLoS One. 2023 Jun 21;18(6):e0286946. doi: 10.1371/journal.pone.0286946. eCollection 2023.
Fatty acids (FA) in follicular fluid (FF) are present in an esterified form [triglycerides, cholesterol esters and phospholipids] or as non-esterified FA, which partly originate from blood. However, a comprehensive comparison of blood vs. FF FA in various lipid classes is missing. The aim of this study was to determine the distribution of the FA composition in each lipid class of serum and FF, and to investigate their mutual correlations. A total of 74 patients undergoing assisted reproductive technology treatment were involved in the study. Both in serum as well as FF, saturated FA and mono-unsaturated FA were predominant in non-esterified FA and triglycerides fractions while poly-unsaturated FA were mainly present in phospholipids and cholesterol esters fractions, although phospholipids also contained high proportions of saturated FA. Irrespective of the lipid class, the FA proportions differed between serum and FF (P < 0.05). Despite these differences, most of the FA in triglycerides, phospholipids and cholesterol esters of FF were well correlated with their proportions in serum. Nevertheless, only weak to moderate associations (r < 0.60) were observed for the majority of the FA in the non-esterified FA fraction. Differences in FA product/precursor-ratios were found between serum and FF, such as higher C20:4n-6 to C18:2n-6 and C20:5n-3 to C18:3n-3 in FF. FA metabolism (e.g. desaturation and elongation) takes place in cells of the intrafollicular micro-environment. Moreover, good correlations between esterified FA in serum and FF suggest esterified FA in blood could be representative of esterified FA in FF.
卵泡液 (FF) 中的脂肪酸 (FA) 以酯化形式 [甘油三酯、胆固醇酯和磷脂] 或非酯化 FA 存在，部分来源于血液。然而，各种脂质类别的血液与 FF FA 之间的综合比较尚缺乏。本研究旨在确定血清和 FF 中各脂质类别的 FA 组成分布，并研究它们之间的相互关系。共有 74 名接受辅助生殖技术治疗的患者参与了这项研究。在血清和 FF 中，饱和 FA 和单不饱和 FA 在非酯化 FA 和甘油三酯中占主导地位，而多不饱和 FA 主要存在于磷脂和胆固醇酯中，尽管磷脂也含有大量的饱和 FA。无论脂质类别如何，血清和 FF 中的 FA 比例均存在差异（P < 0.05）。尽管存在这些差异，但 FF 中甘油三酯、磷脂和胆固醇酯中的大多数 FA 与其在血清中的比例密切相关。然而，非酯化 FA 大部分 FA 的相关性较弱至中度（r < 0.60）。还发现血清和 FF 之间存在 FA 产物/前体比值的差异，例如 FF 中的 C20:4n-6 与 C18:2n-6 和 C20:5n-3 与 C18:3n-3 的比值较高。FA 代谢（例如，去饱和和延伸）发生在卵泡内微环境的细胞中。此外，血清和 FF 中酯化 FA 之间的良好相关性表明，血液中的酯化 FA 可能代表 FF 中的酯化 FA。
