人类和猿猴免疫缺陷病毒蚀斑及中和试验评估
Evaluation of human and simian immunodeficiency virus plaque and neutralization assays.
作者信息
McKeating J A, McKnight A, McIntosh K, Clapham P R, Mulder C, Weiss R A
机构信息
Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.
出版信息
J Gen Virol. 1989 Dec;70 ( Pt 12):3327-33. doi: 10.1099/0022-1317-70-12-3327.
A number of CD4+ T cell lines were compared for their ability to act as target cells for human immunodeficiency virus (HIV) infection in syncytium- and plaque-forming assays. MT-4 and C8166 cells were the most sensitive indicator cells for HIV- and simian immunodeficiency virus (SIV)-induced cytopathic effects, and gave rise to macroscopic (MT-4) and microscopic (C8166) plaques. The MT-4 plaque assay was evaluated for the measurement of HIV- and SIV-neutralizing antibodies.
在细胞融合和噬斑形成试验中，对多个CD4 + T细胞系作为人类免疫缺陷病毒（HIV）感染靶细胞的能力进行了比较。MT - 4和C8166细胞是对HIV和猿猴免疫缺陷病毒（SIV）诱导的细胞病变效应最敏感的指示细胞，并产生肉眼可见的（MT - 4）和显微镜下可见的（C8166）噬斑。对MT - 4噬斑试验进行了评估，以用于测量HIV和SIV中和抗体。
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