Khan A Z, Aarons L
Pharmacy Department, University of Manchester, UK.
J Pharm Pharmacol. 1989 Oct;41(10):710-1. doi: 10.1111/j.2042-7158.1989.tb06346.x.
A sequential approach is presented to the problem of determining the minimum number of blood samples needed to calculate the plasma to saliva concentration ratio to a required precision. The method was applied to salicylate concentrations obtained from six rheumatoid arthritis patients. In order to achieve a 10 per cent coefficient of variation in the plasma to saliva salicylic acid concentration ratio, on average 9 samples were required for total plasma concentration and 8 samples for unbound concentration. In some cases it was not possible to achieve the required precision with the given number of samples. Correlation of salicylic acid concentrations in saliva with total and unbound plasma concentration were equally as good. The limitations of saliva data in clinical pharmacokinetic studies are discussed.
本文提出了一种序贯方法，用于解决确定计算血浆与唾液浓度比至所需精度所需的最少血样数量的问题。该方法应用于从六名类风湿性关节炎患者获得的水杨酸盐浓度。为了使血浆与唾液水杨酸浓度比的变异系数达到10%，平均总血浆浓度需要9个样本，游离浓度需要8个样本。在某些情况下，给定数量的样本无法达到所需的精度。唾液中水杨酸浓度与总血浆浓度和游离血浆浓度的相关性同样良好。文中讨论了临床药代动力学研究中唾液数据的局限性。
