一种从葛根中分离异黄酮衍生物的新颖且有效的色谱方法。
A novel and effective chromatographic approach to the separation of isoflavone derivatives from Pueraria lobata.
作者信息
Fu Jiang, Jing Wenguang, Wang Weihao, Chen Sha, Zhang Jun, Liu An
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
College of Pharmacy, Jiang Xi University of Traditional Chinese Medicine, Nanchang 330006, China.
出版信息
Molecules. 2015 Mar 5;20(3):4238-53. doi: 10.3390/molecules20034238.
A novel and effective chromatographic approach to the separation and purification of isoflavone compounds from Pueraria lobata is described. The method is based on flash chromatography (FC), coupled to preparative high performance liquid chromatography (prep-HPLC) via a six-way valve. The FC step comprised tandem reversed phase columns, pre-packed with MCI gel (Mitsubishi Chemical Corp., Tokyo, Japan) and C18 (Fuji Silysia Chemical Ltd, Osaka, Japan) resin, respectively, and was designed to separate a crude Pueraria lobata extract into several preliminary fractions. Fractions containing the target compounds were then directly injected via the six-way valve into prep-HPLC columns, without further treatment, for final isolation and purification. Nine isoflavonoids were successfully isolated, three through an online mode and the other six through an offline mode. The purities of all compounds exceeded 95.0%, as determined by HPLC with an UV-vis photodiode array detector. The convenience, low solvent consumption, and time-saving advantages of this method offer an attractive and promising approach to the isolation of natural products.
描述了一种用于从葛根中分离和纯化异黄酮化合物的新颖且有效的色谱方法。该方法基于快速色谱法（FC），通过六通阀与制备型高效液相色谱法（prep-HPLC）联用。FC步骤包括串联的反相柱，分别预先填充有MCI凝胶（日本东京三菱化学公司）和C18（日本大阪富士硅化学有限公司）树脂，旨在将粗制的葛根提取物分离成几个初步馏分。然后，含有目标化合物的馏分通过六通阀直接注入prep-HPLC柱中，无需进一步处理，以进行最终的分离和纯化。成功分离出九种异黄酮，其中三种通过在线模式，另外六种通过离线模式。通过配备紫外-可见光电二极管阵列检测器的HPLC测定，所有化合物的纯度均超过95.0%。该方法的便利性、低溶剂消耗和省时优势为天然产物的分离提供了一种有吸引力且有前景的方法。
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