[Function of a calcium-dependent protein kinase gene Pscamk in Puccinia striiformis f. sp. tritici].

OBJECTIVE

To clone calcium-dependent protein kinase gene (camk) from Puccinia striiformis f. sp. tritici (Pst) and analyze its function.

METHODS

The cDNA full-length of Pscamk was isolated by using reverse transcriptional-PCR (RT-PCR), and gene expression profile at different morphological stages was analyzed via quantitative real-time--PCR (qRT-PCR). Pst urediospores were treated with CaMK suppressor KN-93 and germination rate was investigated.

RESULTS

A gene cDNA full-length with 1 620 bp was obtained and designated as Pscamk. qRT-PCR analysis showed Pscamk expression was highly induced in the early stages of Pst infection and reached the maximum at 6 h post inoculation (hpi) as 20.74-fold as that in the control (0 hpi). With increasing of the concentration of CaMK suppressor KN-93, germination rate of Pst urediospores was gradually decreased. The germination rate was reduced to 8.02%, only 12% of the control, under 1.4 μmol/L KN-93 treatment at 10 h after incubation at 9 degrees C.

CONCLUSION

Pscamk might play a role in germination and germ tube elongation of Pst urediospores. This study provides a basis for exploring pathogenesis of calcium signaling pathway during Pst infection.