Belichenko V M, Turganbaeva A S, Khodyrev E V, Kisliakova L P, Kisliakov Iu Ia, Shoshenko K A
Ontogenez. 2014 Sep-Oct;45(5):333-40.
The aim of the study was measure the development chick tissue partial pressure of oxygen (pO2) in the brain cerebral hemispheres, liver, M. pectoralis, M. gastrocnemius, and to estimate the correlation of this index with our previously measured values (laser-Doppler flowmetry) volume blood flow (BF) in these organs. We studied 10-, 15-, and 19-day embryos and 4-day chickens thatwere anesthetized with urethane. pO2 measurements were made in thesurface layers of organs by the membrane amperometric Clark-type O2-electrode with a diameter of about 50 microm of the cathode placed in the center of the sensor with an outer diameter of 3.4 mm. Disclosed noticeable distinction of both the organ tissue pO2 values, and the dynamics of their changes duringthe study period. The most important of them: (1) the lowest pO2 (and BF) is observed in the brain and particularly in the liver of 10-day embryos; (2) in the subsequent period of embryogenesis pO2 in the brain increases 1.9 times (rising and BF) in M. pectoralis it falls by 1.7 times and in the liver.and in M. gastrocnemius changes little wherein the BF in both liver and muscles is not changed, (3) after hatching pO2 in the liver and M. petoralis fold increased (also increasing and BF), and in the brain and M. gastrocnemius, despite the increase BF (greater in the niuscle) did not significant change. In the analysis ofthedata are considered two possible mechanisms of change of tissue pO2 in the developing organs of chickens, one is due to the peculiarities of intracardiac blood flows; and the other is related to thesingularity of the oxyhemoglobin dissociation in the blood capillary bed of organ, determined by the specifics of its oxidative metabolism.
本研究的目的是测量发育中的雏鸡大脑半球、肝脏、胸肌、腓肠肌组织的氧分压（pO2），并估计该指标与我们之前测量的这些器官的体积血流（BF）值（激光多普勒血流仪测量）之间的相关性。我们研究了用氨基甲酸乙酯麻醉的10日龄、15日龄和19日龄胚胎以及4日龄雏鸡。通过将直径约50微米的阴极置于外径为3.4毫米的传感器中心的膜式安培克拉克型O2电极，在器官表层进行pO2测量。结果显示，在研究期间，器官组织的pO2值及其变化动态均存在显著差异。其中最重要的差异包括：（1）在10日龄胚胎的大脑尤其是肝脏中观察到最低的pO2（和BF）；（2）在胚胎发育的后续阶段，大脑中的pO2增加了1.9倍（同时BF上升），胸肌中的pO2下降了1.7倍，肝脏中的pO2变化不大，腓肠肌中的pO2变化也不大，且肝脏和肌肉中的BF均未改变；（3）孵化后，肝脏和胸肌中的pO2翻倍增加（同时BF也增加），而大脑和腓肠肌中的pO2，尽管BF增加（肌肉中增加更多），但没有显著变化。在数据分析中，考虑了雏鸡发育器官中组织pO2变化的两种可能机制，一种是由于心内血流的特殊性；另一种与器官毛细血管床中氧合血红蛋白解离的特殊性有关，这由其氧化代谢的特性决定。
